Modulation of Intestinal Gene Expression by Dietary Zinc Status: Effectiveness of cDNA Arrays for Expression Profiling of a Single Nutrient Deficiency

Mammalian nutritional status affects the homeostatic balance of multiple physiological processes and their associated gene expression. Although DNA array analysis can monitor large numbers of genes, there are no reports of expression profiling of a micronutrient deficiency in an intact animal system...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2001-11, Vol.98 (24), p.13507-13513
Main Authors: Blanchard, Raymond K., Moore, J. Bernadette, Green, Calvert L., Cousins, Robert J.
Format: Article
Language:English
Subjects:
Acrodermatitis
Animals
Biological Sciences
cDNA arrays
Complementary DNA
Deoxyribonucleic acid
Diet
Dietary Supplements
Digestive system
DNA
Gene expression
Gene Expression Profiling
Gene Expression Regulation
Genes
Intestinal Mucosa - metabolism
Male
Messenger RNA
Nutrition
Oligonucleotide Array Sequence Analysis
Rats
Rats, Sprague-Dawley
RNA
Small intestine
uroguanylin
Zinc
Zinc - deficiency
zinc transporter 2
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Summary:Mammalian nutritional status affects the homeostatic balance of multiple physiological processes and their associated gene expression. Although DNA array analysis can monitor large numbers of genes, there are no reports of expression profiling of a micronutrient deficiency in an intact animal system. In this report, we have tested the feasibility of using cDNA arrays to compare the global changes in expression of genes of known function that occur in the early stages of rodent zinc deficiency. The gene-modulating effects of this deficiency were demonstrated by real-time quantitative PCR measurements of altered mRNA levels for metallothionein 1, zinc transporter 2, and uroguanylin, all of which have been previously documented as zinc-regulated genes. As a result of the low level of inherent noise within this model system and application of a recently reported statistical tool for statistical analysis of microarrays [Tusher, V.G., Tibshirani, R. & Chu, G. (2001) Proc. Natl. Acad. Sci. USA 98, 5116-5121], we demonstrate the ability to reproducibly identify the modest changes in mRNA abundance produced by this single micronutrient deficiency. Among the genes identified by this array profile are intestinal genes that influence signaling pathways, growth, transcription, redox, and energy utilization. Additionally, the influence of dietary zinc supply on the expression of some of these genes was confirmed by real-time quantitative PCR. Overall, these data support the effectiveness of cDNA array expression profiling to investigate the pleiotropic effects of specific nutrients and may provide an approach to establishing markers for assessment of nutritional status.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.251532498