Retroviral Delivery of Small Interfering RNA into Primary Cells

RNA interference is an evolutionarily conserved process in which recognition of double-stranded RNA ultimately leads to posttranscriptional suppression of gene expression. This suppression is mediated by short (21- to 22-nt) small interfering RNAs (siRNAs), which induce degradation of mRNA based on...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2002-11, Vol.99 (23), p.14943-14945
Main Authors: Barton, Gregory M., Medzhitov, Ruslan
Format: Article
Language:English
Subjects:
Biological Sciences
Blotting, Southern
Cell Line
Cell lines
Cells
Cytomegalovirus
Double stranded RNA
Fibroblasts
Flow Cytometry
Genetic Vectors
Humans
Inactivation
Mutagenesis, Insertional
Retroviral vectors
Retroviridae - genetics
Ribonucleic acid
RNA
RNA, Small Interfering - genetics
Small interfering RNA
Transfection
Tumor Suppressor Protein p53 - genetics
Viruses
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Summary:RNA interference is an evolutionarily conserved process in which recognition of double-stranded RNA ultimately leads to posttranscriptional suppression of gene expression. This suppression is mediated by short (21- to 22-nt) small interfering RNAs (siRNAs), which induce degradation of mRNA based on complementary base pairing. The silencing of gene expression by siRNAs is emerging rapidly as a powerful method for genetic analysis. Recently, several groups have reported systems designed to express siRNAs in mammalian cells through transfection of either oligonucleotides or plasmids encoding siRNAs. Because these systems rely on transfection for delivery, the cell types available for study are restricted generally to transformed cell lines. Here, we describe a retroviral system for delivery of siRNA into cells. The use of retroviral vectors can greatly expand the types of cells available for RNA interference analysis. Furthermore, we demonstrate that this retroviral system allows for stable inactivation of genes in primary cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.242594499