Infection of a Human Hepatoma Cell Line by Hepatitis B Virus

Among numerous established human hepatoma cell lines, none has been shown susceptible to hepatitis B virus (HBV) infection. We describe here a cell line, called HepaRG, which exhibits hepatocyte-like morphology, expresses specific hepatocyte functions, and supports HBV infection as well as primary c...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2002-11, Vol.99 (24), p.15655-15660
Main Authors: Gripon, Philippe, Rumin, Sylvie, Urban, Stephan, Le Seyec, Jacques, Glaise, Denise, Cannie, Isabelle, Guyomard, Claire, Lucas, Josette, Trepo, Christian, Guguen-Guillouzo, Christiane
Format: Article
Language:English
Subjects:
Antibodies
Biological Sciences
Biomarkers
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - pathology
Carcinoma, Hepatocellular - virology
Cell lines
Cells
Cellular differentiation
Cultured cells
Dimethyl Sulfoxide - pharmacology
DNA
DNA, Viral - isolation & purification
Female
Hep G2 cells
Hepatitis
Hepatitis B
Hepatitis B virus
Hepatitis B virus - growth & development
Hepatitis C - pathology
Hepatocytes
Hepatocytes - virology
Humans
Infections
Karyotyping
Liver - enzymology
Liver Neoplasms - genetics
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
Liver Neoplasms - virology
Microbiology
Organ Specificity
RNA
RNA, Messenger - genetics
RNA, Messenger - isolation & purification
RNA, Viral - isolation & purification
Tumor Cells, Cultured - metabolism
Tumor Cells, Cultured - virology
Virus Cultivation
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Summary:Among numerous established human hepatoma cell lines, none has been shown susceptible to hepatitis B virus (HBV) infection. We describe here a cell line, called HepaRG, which exhibits hepatocyte-like morphology, expresses specific hepatocyte functions, and supports HBV infection as well as primary cultures of normal human hepatocytes. Differentiation and infectability are maintained only when these cells are cultured in the presence of corticoids and dimethyl sulfoxide. The specificity of this HBV infection model was ascertained by both the neutralization capacity of HBV-envelope protein-specific antibodies and the competition with an envelope-derived peptide. HepaRG cells therefore represent a tool for deciphering the mechanism of HBV entry. Moreover, their close resemblance to normal human hepatocytes makes them suitable for many applications including drug metabolism studies.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.232137699