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Infection of a Human Hepatoma Cell Line by Hepatitis B Virus
Among numerous established human hepatoma cell lines, none has been shown susceptible to hepatitis B virus (HBV) infection. We describe here a cell line, called HepaRG, which exhibits hepatocyte-like morphology, expresses specific hepatocyte functions, and supports HBV infection as well as primary c...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 2002-11, Vol.99 (24), p.15655-15660 |
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| cites | cdi_FETCH-LOGICAL-c587t-eeef7a07d3874e19dd2785e5c1daca7a4e6742eae5a3798fc786a3a520e831743 |
| container_end_page | 15660 |
| container_issue | 24 |
| container_start_page | 15655 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 99 |
| creator | Gripon, Philippe Rumin, Sylvie Urban, Stephan Le Seyec, Jacques Glaise, Denise Cannie, Isabelle Guyomard, Claire Lucas, Josette Trepo, Christian Guguen-Guillouzo, Christiane |
| description | Among numerous established human hepatoma cell lines, none has been shown susceptible to hepatitis B virus (HBV) infection. We describe here a cell line, called HepaRG, which exhibits hepatocyte-like morphology, expresses specific hepatocyte functions, and supports HBV infection as well as primary cultures of normal human hepatocytes. Differentiation and infectability are maintained only when these cells are cultured in the presence of corticoids and dimethyl sulfoxide. The specificity of this HBV infection model was ascertained by both the neutralization capacity of HBV-envelope protein-specific antibodies and the competition with an envelope-derived peptide. HepaRG cells therefore represent a tool for deciphering the mechanism of HBV entry. Moreover, their close resemblance to normal human hepatocytes makes them suitable for many applications including drug metabolism studies. |
| doi_str_mv | 10.1073/pnas.232137699 |
| format | article |
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We describe here a cell line, called HepaRG, which exhibits hepatocyte-like morphology, expresses specific hepatocyte functions, and supports HBV infection as well as primary cultures of normal human hepatocytes. Differentiation and infectability are maintained only when these cells are cultured in the presence of corticoids and dimethyl sulfoxide. The specificity of this HBV infection model was ascertained by both the neutralization capacity of HBV-envelope protein-specific antibodies and the competition with an envelope-derived peptide. HepaRG cells therefore represent a tool for deciphering the mechanism of HBV entry. 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Moreover, their close resemblance to normal human hepatocytes makes them suitable for many applications including drug metabolism studies.</description><subject>Antibodies</subject><subject>Biological Sciences</subject><subject>Biomarkers</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Carcinoma, Hepatocellular - virology</subject><subject>Cell lines</subject><subject>Cells</subject><subject>Cellular differentiation</subject><subject>Cultured cells</subject><subject>Dimethyl Sulfoxide - pharmacology</subject><subject>DNA</subject><subject>DNA, Viral - isolation & purification</subject><subject>Female</subject><subject>Hep G2 cells</subject><subject>Hepatitis</subject><subject>Hepatitis B</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus - growth & development</subject><subject>Hepatitis C - pathology</subject><subject>Hepatocytes</subject><subject>Hepatocytes - virology</subject><subject>Humans</subject><subject>Infections</subject><subject>Karyotyping</subject><subject>Liver - enzymology</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver Neoplasms - virology</subject><subject>Microbiology</subject><subject>Organ Specificity</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - isolation & purification</subject><subject>RNA, Viral - isolation & purification</subject><subject>Tumor Cells, Cultured - metabolism</subject><subject>Tumor Cells, Cultured - virology</subject><subject>Virus Cultivation</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkc2P0zAQxS0EYrsLV04IIg6IS4o_M7YEB7YCulIlLsDV8iYTcJXEXdtB7H-Pq5bycYCTpZnfs968R8gjRpeMgni5m1xacsGZgMaYO2TBqGF1Iw29SxaUcqi15PKMnKe0pZQapel9csa4FJwaWJBXV1OPbfZhqkJfuWo9j26q1rhzOYyuWuEwVBs_YXV9e5j67FN1WX32cU4PyL3eDQkfHt8L8und24-rdb358P5q9WZTt0pDrhGxB0ehExokMtN1HLRC1bLOtQ6cxAYkR4fKCTC6b0E3TjjFKWrBQIoL8vrw726-HrFrccrRDXYX_ejirQ3O2z83k_9qv4RvtqQCwIv--VEfw82MKdvRp7ac5iYMc7LAgSvJ6H9BppsSoWQFfPYXuA1znEoIllMmDAO9t708QG0MKUXsT44Ztfv27L49e2qvCJ78fucv_FhXAZ4egb3w59oYy6VlqlGqEC_-Tdh-HoaM33NBHx_QbcohnlhRjGmuxQ88z7ZL</recordid><startdate>20021126</startdate><enddate>20021126</enddate><creator>Gripon, Philippe</creator><creator>Rumin, Sylvie</creator><creator>Urban, Stephan</creator><creator>Le Seyec, Jacques</creator><creator>Glaise, Denise</creator><creator>Cannie, Isabelle</creator><creator>Guyomard, Claire</creator><creator>Lucas, Josette</creator><creator>Trepo, Christian</creator><creator>Guguen-Guillouzo, Christiane</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20021126</creationdate><title>Infection of a Human Hepatoma Cell Line by Hepatitis B Virus</title><author>Gripon, Philippe ; 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We describe here a cell line, called HepaRG, which exhibits hepatocyte-like morphology, expresses specific hepatocyte functions, and supports HBV infection as well as primary cultures of normal human hepatocytes. Differentiation and infectability are maintained only when these cells are cultured in the presence of corticoids and dimethyl sulfoxide. The specificity of this HBV infection model was ascertained by both the neutralization capacity of HBV-envelope protein-specific antibodies and the competition with an envelope-derived peptide. HepaRG cells therefore represent a tool for deciphering the mechanism of HBV entry. Moreover, their close resemblance to normal human hepatocytes makes them suitable for many applications including drug metabolism studies.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>12432097</pmid><doi>10.1073/pnas.232137699</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2002-11, Vol.99 (24), p.15655-15660 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_99_24_15655 |
| source | JSTOR Archival Journals and Primary Sources Collection; PubMed Central |
| subjects | Antibodies Biological Sciences Biomarkers Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Carcinoma, Hepatocellular - virology Cell lines Cells Cellular differentiation Cultured cells Dimethyl Sulfoxide - pharmacology DNA DNA, Viral - isolation & purification Female Hep G2 cells Hepatitis Hepatitis B Hepatitis B virus Hepatitis B virus - growth & development Hepatitis C - pathology Hepatocytes Hepatocytes - virology Humans Infections Karyotyping Liver - enzymology Liver Neoplasms - genetics Liver Neoplasms - metabolism Liver Neoplasms - pathology Liver Neoplasms - virology Microbiology Organ Specificity RNA RNA, Messenger - genetics RNA, Messenger - isolation & purification RNA, Viral - isolation & purification Tumor Cells, Cultured - metabolism Tumor Cells, Cultured - virology Virus Cultivation |
| title | Infection of a Human Hepatoma Cell Line by Hepatitis B Virus |
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