Evaluation of End-Capped DNA Modules for pRNA Capture and Functionalization

The ability of packaging RNA (pRNA) from the phi29 DNA packaging motor to form nanoassemblies and nanostructures has been exploited for the development of the nascent field of RNA nanotechnology and subsequent applications in nanomedicine. For applications in nanomedicine, it is necessary to modify...

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Bibliographic Details
Published in:Bioconjugate chemistry 2012-04, Vol.23 (4), p.683-687
Main Authors: Laing, Brian M, Bergstrom, Donald E
Format: Article
Language:English
Subjects:
Bacillus Phages - genetics
Base Sequence
Deoxyribonucleic acid
DNA
DNA, Viral - chemistry
DNA, Viral - genetics
Molecules
Nanostructured materials
Nanostructures - chemistry
Nucleic Acid Conformation
Nucleic Acid Denaturation
Nucleic Acid Hybridization - methods
Oligodeoxyribonucleotides - chemistry
Oligodeoxyribonucleotides - genetics
Ribonucleic acid
RNA
RNA - chemistry
RNA - genetics
RNA - metabolism
Transition Temperature
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Summary:The ability of packaging RNA (pRNA) from the phi29 DNA packaging motor to form nanoassemblies and nanostructures has been exploited for the development of the nascent field of RNA nanotechnology and subsequent applications in nanomedicine. For applications in nanomedicine, it is necessary to modify the pRNA structure for the conjugation of active molecules. We have investigated end-capped double-stranded DNA segments as reversible capture reagents for pRNA. These capture agents can be designed to allow the conjugation of any desired molecule for pRNA functionalization. The results of model studies presented in this report show that 5- to 7-nucleotide overhangs on a target RNA can provide efficient handles for the high-affinity association to capped double-stranded DNA.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc200371t