VEGF, PF4 and PDGF are elevated in platelets of colorectal cancer patients

Platelets sequester angiogenesis regulatory proteins which suggests an avenue for developing biomarkers to monitor disease. We describe a comparison of angiogenesis regulatory proteins found in platelets of colorectal cancer patients and normal controls. Platelet and plasma content of vascular endot...

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Bibliographic Details
Published in:Angiogenesis (London) 2012-06, Vol.15 (2), p.265-273
Main Authors: Peterson, Jon E., Zurakowski, David, Italiano, Joseph E., Michel, Lea V., Connors, Susan, Oenick, Marsha, D’Amato, Robert J., Klement, Giannoula L., Folkman, Judah
Format: Article
Language:English
Subjects:
Adult
Aged
Biomedical and Life Sciences
Biomedicine
Cancer Research
Cardiology
Cell Biology
Colorectal cancer
Colorectal Neoplasms - blood
Colorectal Neoplasms - pathology
Female
Fibroblast Growth Factor 2 - blood
Humans
Male
Middle Aged
Neovascularization, Pathologic - blood
Neovascularization, Pathologic - pathology
Oncology
Ophthalmology
Original Paper
Platelet Factor 4 - blood
Platelet-Derived Growth Factor - metabolism
Thrombospondin 1 - blood
Vascular Endothelial Growth Factor A - blood
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Summary:Platelets sequester angiogenesis regulatory proteins which suggests an avenue for developing biomarkers to monitor disease. We describe a comparison of angiogenesis regulatory proteins found in platelets of colorectal cancer patients and normal controls. Platelet and plasma content of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), platelet derived growth factor (PDGF), platelet factor 4 (PF4), thrombospondin-1 (TSP-1) and endostatin in 35 patients with colon cancer were compared with 84 age-matched healthy controls using ELISAs. We standardized the platelet preparation procedure, introduced process controls and normalized the respective protein levels to platelet numbers using an actin ELISA. Statistically significant differences were found in the median levels of VEGF, PF4 and PDGF in platelets of patients with cancer compared to healthy individuals. Platelet concentrations in cancer patients versus controls were: VEGF 1.3 versus 0.6 pg/10 6 , PF4 18.5 versus 9.4 ng/10 6 , and PDGF 34.1 versus 21.0 pg/10 6 . Multivariable logistic regression analysis indicated that PDGF, PF4 and VEGF were independent predictors of colorectal carcinoma and as a set provided statistically significant discrimination (area under the curve = 0.893, P  
ISSN:0969-6970
1573-7209
DOI:10.1007/s10456-012-9259-z