Effects of margarine supplemented with T10C12 and C9T11 CLA on atherosclerosis and steatosis in apoE/LDLR -/- mice

• Published in: The Journal of nutrition, health & aging health & aging, 2012-05, Vol.16 (5), p.482-490
• Main Authors: Kostogrys, Renata B, Franczyk-Żarow, M, Maslak, E, Gajda, M, Mateuszuk, Ł, Chłopicki, S
• Format: Article
• Language: English
• Subjects: Aging; Animals; Aorta - pathology; Atherosclerosis; Atherosclerosis (general aspects, experimental research); Atherosclerosis - blood; Atherosclerosis - etiology; Biological and medical sciences; Blood and lymphatic vessels; Body fat; Cardiology. Vascular system; Cholesterol; Cholesterol - blood; conjugated linoleic acid; Diet; Dietary Fats - administration & dosage; Dietary Supplements; elderly; experimental diets; Experiments; fatty liver; Fatty Liver - blood; Fatty Liver - chemically induced; Feeding. Feeding behavior; Food; Food, Fortified; Fundamental and applied biological sciences. Psychology; Geriatrics/Gerontology; Isomerism; isomers; Laboratory animals; Linoleic Acids, Conjugated - adverse effects; Linoleic Acids, Conjugated - chemistry; Linoleic Acids, Conjugated - pharmacology; Lipids; Liver; Liver - drug effects; Liver - pathology; margarine; Margarine - adverse effects; Medical research; Medical sciences; Medicine; Medicine & Public Health; Metabolism; Mice; Mice, Knockout; Neurosciences; Nutrition; Nutrition research; Organ Size; Plaque, Atherosclerotic - pathology; Plasma; Primary Care Medicine; Quality of Life Research; triacylglycerols; Triglycerides - blood; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 1279-7707; 1760-4788
• DOI: 10.1007/s12603-011-0354-4

OBJECTIVES: The objective of this study was to evaluate functional effects of margarine supplemented with individual CLA isomers trans-10, cis-12 and cis-9, trans-11 in apoE/LDLR -/- mice. DESIGN: In LONG experiment (LONG), two-month old mice with no atherosclerosis were assigned to experimental groups and fed for the next 4 months. In SHORT experiment (SHORT), four-month old mice, with pre-established atherosclerosis, were assigned to experimental groups and fed for the next 2 months. The experimental diets were: ALN-93G (margarine), AIN-93G + 0.5% trans-10, cis-12 CLA (tl0cl2), and AIN-93G + 0.5% cis-9, trans-11 CLA (c9tll). RESULTS: In both experiments (LONG and SHORT), liver weight was significantly (P