p53 Hot‐Spot Mutational Analysis in Advanced Western Gallbladder Carcinoma

In gallbladder carcinoma, studies on the prime target of genetic alterations and gene therapy in human gallbladder malignancies, the p53 tumor suppressor gene, have been focusing on this gene's immunohistochemical detection. From November 1991 to October 1993, seven patients suffering from gall...

Full description

Saved in:
Bibliographic Details
Published in:World journal of surgery 1997-09, Vol.21 (7), p.768-772
Main Authors: Jonas, Sven, Springmeier, Giovanni, Tauber, Rudolf, Wiedenmann, Bertram, Gessner, Reinhard, Kling, Norbert, Lobeck, Hartmut, Fieger, Claudia, Bechstein, Wolf O., Neuhaus, Peter
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Aged
Biological and medical sciences
Carcinoma - genetics
Carcinoma, Squamous Cell - genetics
Caucasian Patient
Cloning, Molecular
Codon
DNA Mutational Analysis
DNA, Neoplasm - analysis
European Continental Ancestry Group
Female
Gallbladder Cancer
Gallbladder Carcinoma
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression Regulation, Neoplastic
Genes, p53 - genetics
Humans
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Male
Medical sciences
Middle Aged
Missense Mutation
Mutation
Polymerase Chain Reaction
Tumors
Urinary Bladder Neoplasms - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In gallbladder carcinoma, studies on the prime target of genetic alterations and gene therapy in human gallbladder malignancies, the p53 tumor suppressor gene, have been focusing on this gene's immunohistochemical detection. From November 1991 to October 1993, seven patients suffering from gallbladder carcinoma underwent surgical resection. Cancerous and normal liver tissues were obtained immediately after surgery, snap‐frozen in liquid nitrogen, and stored at −80°C for immunohistochemistry and DNA isolation. Exons 5, 6, 7, and 8 of thep53 gene were completely sequenced following polymerase chain reaction (PCR) amplification of a 1574‐bp fragment. Missense mutations were detected in the cancerous tissues of two patients: one transition each on codons 134 (Phe→Leu) and 146 (Trp→Arg). Immunohistochemical p53 staining was positive in the latter patient only. This is the first report on sequence analysis and mutagenesis of the p53 gene in Caucasian patients with gallbladder cancer. Both mutations were transitions and seem to represent a rather rare event. The possible impact of p53mutagenesis on gallbladder tumorigenesis requires evaluation in larger studies.
ISSN:0364-2313
1432-2323
DOI:10.1007/s002689900303