Influence of nitric oxide on neurogenic contraction and relaxation of the human gastroepiploic artery

• Published in: American journal of hypertension 2003-01, Vol.16 (1), p.28-32
• Main Authors: Medina, Pascual, Segarra, Gloria, Peiro, Marta, Flor, Blas, Martínez-León, Juan B, Vila, José M, Lluch, Salvador
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Anesthetics, Local - pharmacology; Arginine - pharmacology; Autonomic Nervous System - physiology; Biological and medical sciences; Blood vessels and receptors; Endothelial factors; Endothelium, Vascular - metabolism; Enzyme Inhibitors - pharmacology; Female; Fundamental and applied biological sciences. Psychology; Gastroepiploic Artery - innervation; Gastroepiploic Artery - physiology; human gastroepiploic artery; Humans; In Vitro Techniques; Male; Middle Aged; neurotransmission; nitric oxide; Nitric Oxide - metabolism; Nitric Oxide Synthase - antagonists & inhibitors; omega-N-Methylarginine - pharmacology; Tetrodotoxin - pharmacology; Vasoconstriction - drug effects; Vasoconstriction - physiology; Vasodilation - drug effects; Vasodilation - physiology; Vertebrates: cardiovascular system
• ISSN: 0895-7061; 1879-1905; 1941-7225
• DOI: 10.1016/S0895-7061(02)03156-4

The objective of this study was to characterize the neurogenic contraction and relaxation of the human gastroepiploic artery and to determine whether the responses are mediated by nitric oxide (NO) from neural or endothelial origin. Rings of human gastroepiploic artery were obtained from 18 patients (12 men, 6 women) undergoing gastrectomy. The rings were suspended in organ baths for isometric recording of tension. We studied the contractile and relaxant responses to electrical field stimulation. In arteries under resting conditions, electrical field stimulation (2 to 8 Hz) caused frequency-dependent contractions that were of greater magnitude in arteries denuded of endothelium and blocked by tetrodotoxin (10−6 mol/L). The inhibitor of NO synthesis NG-monomethyl-l-arginine (L-NMMA, 10−4 mol/L) increased contractile responses only in arteries with endothelium. In preparations contracted with norepinephrine in the presence of guanethidine (10−6 mol/L) and atropine (10−6 mol/L), electrical stimulation induced frequency-dependent relaxations. This neurogenic relaxation was prevented by L-NMMA (10−4 mol/L) and tetrodotoxin (10−6 mol/L), but was unaffected by removal of the endothelium. The results provide functional evidence that NO is released by autonomic nerves of the human gastroepiploic artery. We hypothesize that the release of NO from both endothelial and neurogenic origin may modulate resistance of the human gastroepiploic artery. Dysfunction in any of these sources of NO should be considered in some form of vasospasm.