Dermatofibrosarcoma protuberans: dealing with slow Mohs procedures employing formalin-fixed, paraffin wax-embedded tissue in a busy diagnostic laboratory

Dermatofibrosarcoma protuberans (DFSP) is a relatively uncommon tumour that arises in the dermis and underlying soft tissue. Surgical removal is the preferred treatment, with relatively wide clearance margins of 3 cm or more. Slow Mohs procedures are often employed successfully to treat patients wit...

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Bibliographic Details
Published in:British journal of biomedical science 2012-01, Vol.69 (2), p.56-61
Main Authors: Orchard, G. E., Shams, M.
Format: Article
Language:English
Subjects:
Antigens, CD34 - biosynthesis
Coloring Agents - pharmacology
Dermatofibrosarcoma - diagnosis
Dermatofibrosarcoma - pathology
Dermatofibrosarcoma - surgery
Dermatofibrosarcoma protuberans
Female
Formaldehyde
Frozen Sections - methods
Histological Techniques - methods
Humans
Immunohistochemistry
Immunohistochemistry - methods
Laboratories
Male
Medical Laboratory Science - methods
Mohs surgery
Mohs Surgery - methods
Neoplasm Metastasis
Paraffin Embedding
Skin Neoplasms - diagnosis
Skin Neoplasms - pathology
Skin Neoplasms - surgery
Tumors
Wound healing
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Description
Summary:Dermatofibrosarcoma protuberans (DFSP) is a relatively uncommon tumour that arises in the dermis and underlying soft tissue. Surgical removal is the preferred treatment, with relatively wide clearance margins of 3 cm or more. Slow Mohs procedures are often employed successfully to treat patients with such tumours. Slow Mohs procedures offer the benefit of improved cure rates and maximal tissue conservation. However, dealing with such tissue successfully presents the laboratory with a host of technical problems. This report advocates a set protocol to follow for slow Mohs, based on the experience acquired from dealing with 37 cases of DFSP over a 12-year period. The report establishes the benefits of slow Mohs paraffin wax-embedded tissue over frozen sections in terms of improved morphology, tissue preservation and immunocytochemical labelling with anti-CD34.
ISSN:0967-4845
2474-0896
DOI:10.1080/09674845.2012.12002437