Development and initial evaluation of diversity array technology for soybean and mungbean

• Published in: Euphytica 2012-08, Vol.186 (3), p.741-754
• Main Authors: Hang Vu, T. T., Lawn, R. J., Bielig, L. M., Molnar, S. J., Xia, L., Kilian, A.
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Analysis; Beans; Biological and medical sciences; Biological diversity; Biomedical and Life Sciences; Biotechnology; Deoxyribonucleic acid; DNA; Ethylenediaminetetraacetic acid; Fundamental and applied biological sciences. Psychology; Genetic diversity; Genetics and breeding of economic plants; Genomics; Legumes; Libraries; Life Sciences; Mimosaceae; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Polymorphism; Povidone; Soybeans; Taxa
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-011-0573-0

Diversity Array Technology (DArT), a technique for quickly generating large numbers of molecular markers, was established for two legume crops, soybean ( Glycine max ) and mungbean ( Vigna radiata ). For each crop, two genomic complexity reduction methods, utilizing Pst I/ Taq I and Pst I/ BstN I restriction digests, were selected for DNA clonal library development and for the isolation in each case of 7,680 DArT clones from genomic representations of pooled DNA samples. While the Pst I/ BstN I method produced more polymorphic clones than Pst I/ Taq I for the soybean library, there was no significant difference between the two methods for the mungbean library. Polymorphism frequencies in mungbean were around twice those in soybean, reflecting greater diversity in the mungbean samples. Even so, there were still nearly 1,500 unique polymorphic clones identified for soybean. The DArT marker transferability from soybean to mungbean (13.6%) was nearly five times higher than that from mungbean to soybean (3.1%). The percentage of DArT marker transferability between mungbean and several other Vigna species ranged from 3.4 to 20.2%. The genetic similarities among 11 diverse Vigna spp. samples, evaluated using the DArT mungbean library, were consistent with published information on these taxa. The results indicated that for soybean and mungbean, the DArT technique is an effective tool for marker generation in terms of speed and the numbers of markers identified. The transferability of markers between soybean and mungbean indicated that DArT may be useful for comparative genomic studies, while the ability of the mungbean library to discriminate between related Vigna taxa suggested that DArT may also be useful for studies of genetic diversity.