Highly Photostable Near-Infrared Fluorescent pH Indicators and Sensors Based on BF^sub 2^-Chelated Tetraarylazadipyrromethene Dyes

In this study, a series of new BF2-chelated tetraarylazadipyrromethane dyes are synthesized and are shown to be suitable for the preparation of on/off photoinduced electron transfer modulated fluorescent sensors. The new indicators are noncovalently entrapped in polyurethane hydrogel D4 and feature...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2012-08, Vol.84 (15), p.6723
Main Authors: Jokic, Tijana, Borisov, Sergey M, Saf, Robert, Nielsen, Daniel A, Kühl, Michael, Klimant, Ingo
Format: Article
Language:English
Subjects:
Adaptation
Electron transfer
Fluorescence
Sensors
Online Access:Get full text
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Summary:In this study, a series of new BF2-chelated tetraarylazadipyrromethane dyes are synthesized and are shown to be suitable for the preparation of on/off photoinduced electron transfer modulated fluorescent sensors. The new indicators are noncovalently entrapped in polyurethane hydrogel D4 and feature absorption maxima in the range 660-710 nm and fluorescence emission maxima at 680-740 nm. Indicators have high molar absorption coefficients of 80 000 M... cm..., good quantum yields (up to 20%), excellent photostability and low cross-sensitivity to the ionic strength. ... values of indicators are determined from absorbance and fluorescence measurements and range from 7 to 11, depending on the substitution pattern of electron-donating and -withdrawing functionalities. Therefore, the new indicators are suitable for exploitation and adaptation in a diverse range of analytical applications. Apparent ... values in sensor films derived from fluorescence data show 0.5-1 pH units lower values in comparison with those derived from the absorption data due to Forster resonance energy transfer from protonated to deprotonated form. A dual-lifetime referenced sensor is prepared, and application for monitoring of pH in corals is demonstrated. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0003-2700
1520-6882