Loading…
Abnormal expression of pRb, p16, and cyclin D1 in gastric adenocarcinoma and its lymph node metastases: relationship with pathological features and survival
The retinoblastoma (Rb) pathway controls the G1-S checkpoint of the cell cycle. Inactivating mutations and deletions of p16 and Rb and up-regulation of cyclin D1 disrupt this pathway and occur in many cancers. However, the concurrent expression of these genes in primary and metastatic gastric cancer...
Saved in:
| Published in: | Human pathology 2003-12, Vol.34 (12), p.1276-1282 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c419t-97cb8a3fc4a04a60defb9a2e5a2ce595c1324d42318c6ea0e67ab2f869acf45c3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c419t-97cb8a3fc4a04a60defb9a2e5a2ce595c1324d42318c6ea0e67ab2f869acf45c3 |
| container_end_page | 1282 |
| container_issue | 12 |
| container_start_page | 1276 |
| container_title | Human pathology |
| container_volume | 34 |
| creator | Feakins, Roger M Nickols, Carole D Bidd, Heena Walton, Sarah-Jane |
| description | The retinoblastoma (Rb) pathway controls the G1-S checkpoint of the cell cycle. Inactivating mutations and deletions of p16 and Rb and up-regulation of cyclin D1 disrupt this pathway and occur in many cancers. However, the concurrent expression of these genes in primary and metastatic gastric cancer is unknown, and the prognostic value of their expression is unclear. In this study, the expression of cyclin D1, retinoblastoma protein (pRb), and p16 in 67 resected gastric adenocarcinomas, and of pRb and p16 in 40 associated lymph node metastases, was determined using a streptavidin-biotin-peroxidase immunohistochemical method. Relationships with clinical and pathological features were analyzed. Cyclin D1 overexpression (≥5% expression) was seen in 55% of cancers; pRb loss ( |
| doi_str_mv | 10.1016/j.humpath.2003.07.005 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1034843804</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0046817703004726</els_id><sourcerecordid>2743328421</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-97cb8a3fc4a04a60defb9a2e5a2ce595c1324d42318c6ea0e67ab2f869acf45c3</originalsourceid><addsrcrecordid>eNqFkc2O1DAQhC0EYoeFRwBZQtw2wY7t_HBBq-VXWgkJwdnqOJ2NR4kd7GRg3oWHxbMTaY9cui9fV5W6CHnJWc4ZL9_u82GdZliGvGBM5KzKGVOPyI4rUWS1aIrHZMeYLLOaV9UFeRbjnjHOlVRPyQWXZcMbLnbk73XrfJhgpPhnDhij9Y76ns7f2ys68_KKguuoOZrROvqB0zTvIC7BGgodOm8gGOv8BPecXSIdj9M8UOc7pBMuiYWI8R0NOMKSxONgZ_rbLgM9hfejv7MmufcIy5r872XiGg72AONz8qSHMeKLbV-Sn58-_rj5kt1--_z15vo2M5I3S9ZUpq1B9EYCk1CyDvu2gQIVFAZVowwXhexkIXhtSgSGZQVt0ddlA6aXyohL8vqsOwf_a8W46L1fg0uWmjMhaylqJhOlzpQJPsaAvZ6DnSAcE6RPnei93jrRp040q3TqJN292tTXdsLu4WorIQFvNgBi-kUfwBkbHzglqlqIU4D3Zw7TLw4Wg47GojPY2YBm0Z23_4nyDxwUr_Y</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1034843804</pqid></control><display><type>article</type><title>Abnormal expression of pRb, p16, and cyclin D1 in gastric adenocarcinoma and its lymph node metastases: relationship with pathological features and survival</title><source>ScienceDirect Freedom Collection</source><creator>Feakins, Roger M ; Nickols, Carole D ; Bidd, Heena ; Walton, Sarah-Jane</creator><creatorcontrib>Feakins, Roger M ; Nickols, Carole D ; Bidd, Heena ; Walton, Sarah-Jane</creatorcontrib><description>The retinoblastoma (Rb) pathway controls the G1-S checkpoint of the cell cycle. Inactivating mutations and deletions of p16 and Rb and up-regulation of cyclin D1 disrupt this pathway and occur in many cancers. However, the concurrent expression of these genes in primary and metastatic gastric cancer is unknown, and the prognostic value of their expression is unclear. In this study, the expression of cyclin D1, retinoblastoma protein (pRb), and p16 in 67 resected gastric adenocarcinomas, and of pRb and p16 in 40 associated lymph node metastases, was determined using a streptavidin-biotin-peroxidase immunohistochemical method. Relationships with clinical and pathological features were analyzed. Cyclin D1 overexpression (≥5% expression) was seen in 55% of cancers; pRb loss (<20% expression), in 33%; p16 loss (<10% expression), in 49%; and at least 1 of these abnormalities, in 92.5%. Cyclin D1 overexpression was associated with poor differentiation (
P = 0.027) and signet ring cell type (
P = 0.029). pRb expression was lower in lymph node metastases than in the corresponding primary tumors (
P <0.001). Univariate and multivariate survival analysis (minimum follow-up 72 months or until death) revealed that <20% pRb expression, <30% pRb expression, and International Union Against Cancer stage >2 were associated with worse overall survival. The results suggest that Rb pathway disturbances play an important role in gastric carcinogenesis. The poor prognosis of cancers with low pRb expression and the reduced pRb expression in lymph node metastases raise the possibility that Rb and related genes also influence progression.</description><identifier>ISSN: 0046-8177</identifier><identifier>EISSN: 1532-8392</identifier><identifier>DOI: 10.1016/j.humpath.2003.07.005</identifier><identifier>PMID: 14691913</identifier><identifier>CODEN: HPCQA4</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adenocarcinoma - mortality ; Adenocarcinoma - pathology ; Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Biomarkers, Tumor - analysis ; Biotechnology ; Breast cancer ; Cell cycle ; cyclin D1 ; Cyclin D1 - biosynthesis ; Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis ; Cyclin-dependent kinases ; Female ; gastric carcinoma ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene Expression Regulation, Neoplastic ; Humans ; Immunohistochemistry ; Lymphatic Metastasis - pathology ; Male ; Medical sciences ; Middle Aged ; Mortality ; Multivariate analysis ; p16 ; Pathogenesis ; Prognosis ; Proteins ; retinoblastoma protein ; Retinoblastoma Protein - biosynthesis ; Stomach Neoplasms - mortality ; Stomach Neoplasms - pathology ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Studies ; Survival analysis ; Tumors</subject><ispartof>Human pathology, 2003-12, Vol.34 (12), p.1276-1282</ispartof><rights>2003 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-97cb8a3fc4a04a60defb9a2e5a2ce595c1324d42318c6ea0e67ab2f869acf45c3</citedby><cites>FETCH-LOGICAL-c419t-97cb8a3fc4a04a60defb9a2e5a2ce595c1324d42318c6ea0e67ab2f869acf45c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15378334$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14691913$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feakins, Roger M</creatorcontrib><creatorcontrib>Nickols, Carole D</creatorcontrib><creatorcontrib>Bidd, Heena</creatorcontrib><creatorcontrib>Walton, Sarah-Jane</creatorcontrib><title>Abnormal expression of pRb, p16, and cyclin D1 in gastric adenocarcinoma and its lymph node metastases: relationship with pathological features and survival</title><title>Human pathology</title><addtitle>Hum Pathol</addtitle><description>The retinoblastoma (Rb) pathway controls the G1-S checkpoint of the cell cycle. Inactivating mutations and deletions of p16 and Rb and up-regulation of cyclin D1 disrupt this pathway and occur in many cancers. However, the concurrent expression of these genes in primary and metastatic gastric cancer is unknown, and the prognostic value of their expression is unclear. In this study, the expression of cyclin D1, retinoblastoma protein (pRb), and p16 in 67 resected gastric adenocarcinomas, and of pRb and p16 in 40 associated lymph node metastases, was determined using a streptavidin-biotin-peroxidase immunohistochemical method. Relationships with clinical and pathological features were analyzed. Cyclin D1 overexpression (≥5% expression) was seen in 55% of cancers; pRb loss (<20% expression), in 33%; p16 loss (<10% expression), in 49%; and at least 1 of these abnormalities, in 92.5%. Cyclin D1 overexpression was associated with poor differentiation (
P = 0.027) and signet ring cell type (
P = 0.029). pRb expression was lower in lymph node metastases than in the corresponding primary tumors (
P <0.001). Univariate and multivariate survival analysis (minimum follow-up 72 months or until death) revealed that <20% pRb expression, <30% pRb expression, and International Union Against Cancer stage >2 were associated with worse overall survival. The results suggest that Rb pathway disturbances play an important role in gastric carcinogenesis. The poor prognosis of cancers with low pRb expression and the reduced pRb expression in lymph node metastases raise the possibility that Rb and related genes also influence progression.</description><subject>Adenocarcinoma - mortality</subject><subject>Adenocarcinoma - pathology</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Cell cycle</subject><subject>cyclin D1</subject><subject>Cyclin D1 - biosynthesis</subject><subject>Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis</subject><subject>Cyclin-dependent kinases</subject><subject>Female</subject><subject>gastric carcinoma</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Lymphatic Metastasis - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mortality</subject><subject>Multivariate analysis</subject><subject>p16</subject><subject>Pathogenesis</subject><subject>Prognosis</subject><subject>Proteins</subject><subject>retinoblastoma protein</subject><subject>Retinoblastoma Protein - biosynthesis</subject><subject>Stomach Neoplasms - mortality</subject><subject>Stomach Neoplasms - pathology</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Studies</subject><subject>Survival analysis</subject><subject>Tumors</subject><issn>0046-8177</issn><issn>1532-8392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkc2O1DAQhC0EYoeFRwBZQtw2wY7t_HBBq-VXWgkJwdnqOJ2NR4kd7GRg3oWHxbMTaY9cui9fV5W6CHnJWc4ZL9_u82GdZliGvGBM5KzKGVOPyI4rUWS1aIrHZMeYLLOaV9UFeRbjnjHOlVRPyQWXZcMbLnbk73XrfJhgpPhnDhij9Y76ns7f2ys68_KKguuoOZrROvqB0zTvIC7BGgodOm8gGOv8BPecXSIdj9M8UOc7pBMuiYWI8R0NOMKSxONgZ_rbLgM9hfejv7MmufcIy5r872XiGg72AONz8qSHMeKLbV-Sn58-_rj5kt1--_z15vo2M5I3S9ZUpq1B9EYCk1CyDvu2gQIVFAZVowwXhexkIXhtSgSGZQVt0ddlA6aXyohL8vqsOwf_a8W46L1fg0uWmjMhaylqJhOlzpQJPsaAvZ6DnSAcE6RPnei93jrRp040q3TqJN292tTXdsLu4WorIQFvNgBi-kUfwBkbHzglqlqIU4D3Zw7TLw4Wg47GojPY2YBm0Z23_4nyDxwUr_Y</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>Feakins, Roger M</creator><creator>Nickols, Carole D</creator><creator>Bidd, Heena</creator><creator>Walton, Sarah-Jane</creator><general>Elsevier Inc</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope></search><sort><creationdate>20031201</creationdate><title>Abnormal expression of pRb, p16, and cyclin D1 in gastric adenocarcinoma and its lymph node metastases: relationship with pathological features and survival</title><author>Feakins, Roger M ; Nickols, Carole D ; Bidd, Heena ; Walton, Sarah-Jane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-97cb8a3fc4a04a60defb9a2e5a2ce595c1324d42318c6ea0e67ab2f869acf45c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adenocarcinoma - mortality</topic><topic>Adenocarcinoma - pathology</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Biotechnology</topic><topic>Breast cancer</topic><topic>Cell cycle</topic><topic>cyclin D1</topic><topic>Cyclin D1 - biosynthesis</topic><topic>Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis</topic><topic>Cyclin-dependent kinases</topic><topic>Female</topic><topic>gastric carcinoma</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Lymphatic Metastasis - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mortality</topic><topic>Multivariate analysis</topic><topic>p16</topic><topic>Pathogenesis</topic><topic>Prognosis</topic><topic>Proteins</topic><topic>retinoblastoma protein</topic><topic>Retinoblastoma Protein - biosynthesis</topic><topic>Stomach Neoplasms - mortality</topic><topic>Stomach Neoplasms - pathology</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Studies</topic><topic>Survival analysis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feakins, Roger M</creatorcontrib><creatorcontrib>Nickols, Carole D</creatorcontrib><creatorcontrib>Bidd, Heena</creatorcontrib><creatorcontrib>Walton, Sarah-Jane</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Human pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feakins, Roger M</au><au>Nickols, Carole D</au><au>Bidd, Heena</au><au>Walton, Sarah-Jane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abnormal expression of pRb, p16, and cyclin D1 in gastric adenocarcinoma and its lymph node metastases: relationship with pathological features and survival</atitle><jtitle>Human pathology</jtitle><addtitle>Hum Pathol</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>34</volume><issue>12</issue><spage>1276</spage><epage>1282</epage><pages>1276-1282</pages><issn>0046-8177</issn><eissn>1532-8392</eissn><coden>HPCQA4</coden><abstract>The retinoblastoma (Rb) pathway controls the G1-S checkpoint of the cell cycle. Inactivating mutations and deletions of p16 and Rb and up-regulation of cyclin D1 disrupt this pathway and occur in many cancers. However, the concurrent expression of these genes in primary and metastatic gastric cancer is unknown, and the prognostic value of their expression is unclear. In this study, the expression of cyclin D1, retinoblastoma protein (pRb), and p16 in 67 resected gastric adenocarcinomas, and of pRb and p16 in 40 associated lymph node metastases, was determined using a streptavidin-biotin-peroxidase immunohistochemical method. Relationships with clinical and pathological features were analyzed. Cyclin D1 overexpression (≥5% expression) was seen in 55% of cancers; pRb loss (<20% expression), in 33%; p16 loss (<10% expression), in 49%; and at least 1 of these abnormalities, in 92.5%. Cyclin D1 overexpression was associated with poor differentiation (
P = 0.027) and signet ring cell type (
P = 0.029). pRb expression was lower in lymph node metastases than in the corresponding primary tumors (
P <0.001). Univariate and multivariate survival analysis (minimum follow-up 72 months or until death) revealed that <20% pRb expression, <30% pRb expression, and International Union Against Cancer stage >2 were associated with worse overall survival. The results suggest that Rb pathway disturbances play an important role in gastric carcinogenesis. The poor prognosis of cancers with low pRb expression and the reduced pRb expression in lymph node metastases raise the possibility that Rb and related genes also influence progression.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>14691913</pmid><doi>10.1016/j.humpath.2003.07.005</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0046-8177 |
| ispartof | Human pathology, 2003-12, Vol.34 (12), p.1276-1282 |
| issn | 0046-8177 1532-8392 |
| language | eng |
| recordid | cdi_proquest_journals_1034843804 |
| source | ScienceDirect Freedom Collection |
| subjects | Adenocarcinoma - mortality Adenocarcinoma - pathology Adult Aged Aged, 80 and over Biological and medical sciences Biomarkers, Tumor - analysis Biotechnology Breast cancer Cell cycle cyclin D1 Cyclin D1 - biosynthesis Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis Cyclin-dependent kinases Female gastric carcinoma Gastroenterology. Liver. Pancreas. Abdomen Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Lymphatic Metastasis - pathology Male Medical sciences Middle Aged Mortality Multivariate analysis p16 Pathogenesis Prognosis Proteins retinoblastoma protein Retinoblastoma Protein - biosynthesis Stomach Neoplasms - mortality Stomach Neoplasms - pathology Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Studies Survival analysis Tumors |
| title | Abnormal expression of pRb, p16, and cyclin D1 in gastric adenocarcinoma and its lymph node metastases: relationship with pathological features and survival |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T02%3A50%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Abnormal%20expression%20of%20pRb,%20p16,%20and%20cyclin%20D1%20in%20gastric%20adenocarcinoma%20and%20its%20lymph%20node%20metastases:%20relationship%20with%20pathological%20features%20and%20survival&rft.jtitle=Human%20pathology&rft.au=Feakins,%20Roger%20M&rft.date=2003-12-01&rft.volume=34&rft.issue=12&rft.spage=1276&rft.epage=1282&rft.pages=1276-1282&rft.issn=0046-8177&rft.eissn=1532-8392&rft.coden=HPCQA4&rft_id=info:doi/10.1016/j.humpath.2003.07.005&rft_dat=%3Cproquest_cross%3E2743328421%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c419t-97cb8a3fc4a04a60defb9a2e5a2ce595c1324d42318c6ea0e67ab2f869acf45c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1034843804&rft_id=info:pmid/14691913&rfr_iscdi=true |