Expression of anaphase-promoting complex7 in fibroadenomas and phyllodes tumors of breast

• Published in: Human pathology 2009, Vol.40 (1), p.98-107
• Main Authors: Kang, Yup, PhD, Kim, Jang Hee, MD, Lee, Tae Hui, MD, Kim, Tai Seung, MD, PhD, Jung, Woo Hee, MD, PhD, Chung, Hyun Cheol, MD, PhD, Park, Byeong Woo, MD, PhD, Sheen, Seung Soo, MD, PhD, Han, Jae Ho, MD, PhD
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Anaphase-promoting complex; Anaphase-Promoting Complex-Cyclosome; Biological and medical sciences; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell cycle; Child; Child, Preschool; Cyclin-dependent kinases; Female; Fibroadenoma; Fibroadenoma - genetics; Fibroadenoma - metabolism; Fibroadenoma - pathology; Flow Cytometry; Gene Expression Regulation, Neoplastic; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemistry; Investigative techniques, diagnostic techniques (general aspects); Ki-67; Ki-67 Antigen - metabolism; Labeling; Mammary gland diseases; Medical sciences; Middle Aged; Pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Phase transitions; Phyllodes tumor; Phyllodes Tumor - genetics; Phyllodes Tumor - metabolism; Phyllodes Tumor - pathology; Polyclonal antibodies; Proteins; Retrospective Studies; S Phase; Tumors; Ubiquitin-Protein Ligase Complexes - genetics; Ubiquitin-Protein Ligase Complexes - metabolism; Young Adult
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1016/j.humpath.2008.04.023

Summary The proteolytic destruction of cyclin B is an important event during cell division. Cyclin B proteolysis is triggered by the anaphase-promoting complex. Therefore, cell cycle dysregulation due to anaphase-promoting complex loss contributes to cell transformation and human carcinogenesis. This study investigates anaphase-promoting complex7 expression in spindle cell breast tumors and also includes a comparison between the proliferation antigen Ki-67 and S-phase fraction. The average values of the anaphase-promoting complex7 and Ki-67 labeling indices increased in order from benign to malignant within the phyllodes tumor group, and the fibroadenoma and juvenile fibroadenoma exhibited lower levels of anaphase-promoting complex7 and Ki-67 expression than did the phyllodes tumor. The frequency of anaphase-promoting complex7–positive stromal cells correlated with Ki-67 expression in phyllodes tumor and in all of the examined breast tumors. The above results indicate that anaphase-promoting complex7 and Ki-67 are closely related to cell proliferation. In addition, phyllodes tumor can be differentiated from juvenile fibroadenoma with increased mitotic figures mimicking phyllodes tumor by anaphase-promoting complex7 and Ki-67 immunochemistry. Because anaphase-promoting complex7 is expressed at higher levels than is Ki-67, it may overcome the limitations of the Ki-67 labeling index with regard to the differentiation of benign phyllodes tumor from fibroadenoma.