Imprint cytology detects floating Brachyspira in human intestinal spirochetosis

• Published in: Human pathology 2010-02, Vol.41 (2), p.249-254
• Main Authors: Ogata, Sho, MD, PhD, Higashiyama, Masaaki, MD, Adachi, Yoshikazu, DVM, PhD, Ohara, Ichiyo, MD, PhD, Nishiyama, Junichiro, MD, PhD, Okusa, Yasushi, MD, PhD, Takeo, Hiroaki, MD, PhD, Sato, Kimiya, MD, PhD, Nakanishi, Kuniaki, MD, PhD, Kawai, Toshiaki, MD, PhD
• Format: Article
• Language: English
• Subjects: Adult; Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Biological and medical sciences; Biopsy; Brachyspira; Brachyspira - genetics; Brachyspira - isolation & purification; Colon; Colon - microbiology; Colon - pathology; Colon - ultrastructure; Cytological Techniques; Cytology; DNA, Bacterial - genetics; Genotype; Gram-Negative Bacterial Infections - genetics; Gram-Negative Bacterial Infections - microbiology; Gram-Negative Bacterial Infections - pathology; Histology; Human bacterial diseases; Humans; Hypotheses; Infectious diseases; Intestinal Diseases - genetics; Intestinal Diseases - microbiology; Intestinal Diseases - pathology; Intestinal Mucosa - microbiology; Intestinal Mucosa - pathology; Intestinal Mucosa - ultrastructure; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Methods; Microscopy; Middle Aged; Mucus - microbiology; Pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction; Ribosomal DNA; Spirochetosis; Studies
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1016/j.humpath.2009.07.020

Summary Human intestinal spirochetosis is a colorectal infectious disease caused by 2 Brachyspira species. Its diagnosis is established by histology, culture, and polymerase chain reaction, but the value of cytologic examination in routine practice remains unclear. In this study, imprint cytology of biopsy specimens was examined for cytologic features specific to human intestinal spirochetosis. Specimens were obtained from 65 colorectal regions (1–3 regions from each case) in 25 ultrastructurally and/or genetically confirmed human intestinal spirochetosis cases (20 with Brachyspira aalborgi , 3 with B pilosicoli , 2 with both genotypes). In cytologic specimens, spirochetes tended to be floating freely within the mucus and intestinal fluid, whereas the “fringe formation” of spirochetes typically observed in histologic specimens was indistinct in cytologic specimens. Spirochetes were identified in 58 regions (89.2%) and 23 cases (92.0%) by cytology, against in 50 regions (76.9%) and 22 cases (88.0%) by histology (no significant differences). In 6 of 8 regions exhibiting positive cytology and negative histology, B pilosicoli was present within the mucus. Hence, B pilosicoli may tend to float in the mucus. In conclusion, cytologic examination would be useful for the routine identification of human intestinal spirochetosis, especially if B pilosicoli is involved. Further, we suggest the existence of differences in biological behavior between these spirochetes.