Genome re-arrangements associated with loss of pathogenicity of the [gamma]-herpesvirus alcelaphine herpesvirus-1

The alcelaphine herpesvirus 1 (AlHV-1) causes malignant catarrhal fever in ruminants. Previous work had shown that serial passage of AlHV-1 in culture resulted in genome alterations that are associated with a loss in pathogenicity. Here we have analysed the re-arrangements that occur in more detail....

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Bibliographic Details
Published in:Research in veterinary science 2003-10, Vol.75 (2), p.163
Main Authors: Wright, H, Stewart, J.P, Ireri, R.G, Campbell, I, Pow, I, Reid, H.W, Haig, D.M
Format: Article
Language:English
Subjects:
Cloning
Colleges & universities
Deoxyribonucleic acid
DNA
Genes
Genomes
Rabbits
Veterinary medicine
Viruses
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Summary:The alcelaphine herpesvirus 1 (AlHV-1) causes malignant catarrhal fever in ruminants. Previous work had shown that serial passage of AlHV-1 in culture resulted in genome alterations that are associated with a loss in pathogenicity. Here we have analysed the re-arrangements that occur in more detail. None of the observed re-arrangements was entirely consistent. However, they did all involve translocation of a similar region of DNA from around the centre of the genome to areas either next to or in between terminal repeat elements at either end of the genome. There was also a concomitant loss of the wild-type locus. These re-arrangements appeared to be associated with the loss of virulence and the appearance of cell-free virus.
ISSN:0034-5288
1532-2661
DOI:10.1016/S0034-5288(03)00043-2