Rapid detection of escherichia coli O157 : H7 by fluorescent amplification-based specific hybridization (FLASH) PCR

Background : Escherichia coli O157 : H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. Methods : We developed fluorescent amplification-based specific hybridization (FLASH–PC...

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Bibliographic Details
Published in:Iranian red crescent medical journal 2012, Vol.14 (9), p.1
Main Authors: Khatami, F., Khatami, M., Haydari, M.
Format: Article
Language:English
Subjects:
Escherichia coli infections
Escherichia coli O157:H7
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Summary:Background : Escherichia coli O157 : H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. Methods : We developed fluorescent amplification-based specific hybridization (FLASH–PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157 : H7. Result : PCR product of 336 bp was successfully amplified in a FLASH–PCR. Conclusion : As rapid detection and confirmation of the presence of E. coli O157 : H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E. coli O157 : H7.
ISSN:2074-1804
2074-1812