[alpha]-Ketoglutaric acid production by Yarrowia lipolytica and its regulation

The yeast Yarrowia lipolytica VKM Y-2412 was selected as a prospective producer of α-ketoglutaric acid (KGA) from ethanol. The following peculiarities were found: (1) the intensive KGA production occurred only under the limitation of cell growth by thiamine and the excess of ethanol and nitrogen, (2...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2012-11, Vol.96 (3), p.783
Main Authors: Kamzolova, Svetlana V, Chiglintseva, Maria N, Lunina, Julia N, Morgunov, Igor G
Format: Article
Language:English
Subjects:
Acid production
Analysis
Biochemistry
Biomass
Carbon
Cell growth
Chemical synthesis
Ethanol
Experiments
Fungi
Metabolism
Microbiology
Microorganisms
Nitrogen
Physiology
Studies
Trace elements
Vitamin B
Yeast
Yeasts
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Summary:The yeast Yarrowia lipolytica VKM Y-2412 was selected as a prospective producer of α-ketoglutaric acid (KGA) from ethanol. The following peculiarities were found: (1) the intensive KGA production occurred only under the limitation of cell growth by thiamine and the excess of ethanol and nitrogen, (2) the production of KGA from ethanol required increased amount of zinc and iron ions, and (3) KGA production increased significantly with a high aeration at pH medium equal to 3.5. Under optimal conditions, the Y. lipolytica VKM Y-2412 produced up to 172 gl^sup -1^ of KGA with the mass yield coefficient of 0.70 gg^sup -1^.[PUBLICATION ABSTRACT]
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-012-4222-x