Characterization of a patch-clamp microchannel array towards neuronal networks analysis

• Published in: Microfluidics and nanofluidics 2010-10, Vol.9 (4-5), p.963-972
• Main Authors: Alberti, Massimo, Snakenborg, Detlef, Lopacinska, Joanna M., Dufva, Martin, Kutter, Jörg P.
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Biological and medical sciences; Biomedical Engineering and Bioengineering; Biosensors; Biotechnology; Engineering; Engineering Fluid Dynamics; Fabrication; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Nanotechnology and Microengineering; Research Paper; Silicon; Various methods and equipments
• ISSN: 1613-4982; 1613-4990
• DOI: 10.1007/s10404-010-0619-y

The attempt to combine the planar patch clamping idea with the microelectrode array (MEA) concept has led to the fabrication of a patch clamp microchannel array (PCμCA). Such a system is thought to be a powerful framework for neuroscience research and drug screening, as a novel tool for simultaneous patch clamping of cultured cells or neurons in the same network. A disposable silicon/silicon dioxide (Si/SiO 2 ) chip with a microhole array was integrated in a microfluidic system for cell handling, perfusion and electrical recording. Fluidic characterization showed that our PCμCA can work as a precise local perfusion system for chemicals or drugs. Electrical characterization for microholes of 2 μm and 3 μm revealed an access resistance of 8.09 ± 0.84 MΩ and 3.18 ± 0.63 MΩ, respectively. The capacitance was 98.6 ± 13.2 pF. The values are close to what can be expected from theory, but the capacitance is still too high for high resolution recording. The system was tested on HeLa cells: successful cell trapping with a sealing of 40 MΩ was recorded. Modification of the Si/SiO 2 chip is needed in order to achieve a better sealing and long-term cell culturing in the PCμCA remains to be tested.