In vitro effects of l-arginine on motion parameters in ram epididymal sperm

This study investigated concentration/time-dependent effects of l-arginine on motion parameters of epididymal ram sperm in vitro. After incubation of epididymal sperm samples for 45 and 90 min in the presence of l-arginine (0.001, 0.01, 0.1, and 1 mM), motion parameters were evaluated by computer-ai...

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Bibliographic Details
Published in:Comparative clinical pathology 2010, Vol.19 (4), p.351-355
Main Authors: Hassanpour, Hossein, Teshfam, Masood, Karimi Goodarzi, Ali, Tajik, Parviz, Mirshokraei, Pezhman
Format: Article
Language:English
Subjects:
Hematology
Kinematic parameters
l-arginine
Medicine
Medicine & Public Health
Motility
Oncology
Original Article
Pathology
rams
Sperm
spermatozoa
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Summary:This study investigated concentration/time-dependent effects of l-arginine on motion parameters of epididymal ram sperm in vitro. After incubation of epididymal sperm samples for 45 and 90 min in the presence of l-arginine (0.001, 0.01, 0.1, and 1 mM), motion parameters were evaluated by computer-aided sperm analysis. Parameters of rapid progressive motility, slow progressive motility, non-progressive motility, and immotility did not show any significant effects at the four concentrations of l-arginine after 45 and 90 min. Three motion parameters, straightness (at 45 min), curvilinear velocity, and straight-line velocity (at 90 min) were significantly (P < 0.05) decreased at an l-arginine concentration of 1 mM when compared with controls. Most other motion parameters such as average path velocity, mean angular displacement, amplitude of lateral head displacement, and linearity showed non-significant reduction at an l-arginine concentration of 1 mM at both 45 and 90 min; whereas, wobble and beat cross frequency showed a non-significant increase. In conclusion, low concentrations of l-arginine had little effect on sperm motion parameters; whereas, high concentration of l-arginine significantly decreased specific motion parameters.
ISSN:1618-5641
1618-565X
DOI:10.1007/s00580-009-0876-x