Effects of NOC-18 (nitric oxide donor) and TRIM (nitric oxide synthase inhibitor) on kinematic parameters of ram epididymal sperm

The present study investigated effects of NOC-18 and TRIM on kinematic parameters of epididymal ram sperm in vitro. After incubation of epididymal sperm samples for 45 and 90 min in the presence of NOC-18 (0.0005, 0.5, and 500 µM) and TRIM (0.1, 10, and 1000 µM), motion parameters were evaluated by...

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Bibliographic Details
Published in:Comparative clinical pathology 2010, Vol.19 (5), p.487-491
Main Authors: Hassanpour, Hossein, Teshfam, Masood, Goodarzi, Ali Karimi
Format: Article
Language:English
Subjects:
Fertility
Hematology
Kinematic parameters
Medicine
Medicine & Public Health
Motility
Nitric oxide
NOC-18
Oncology
Original Article
Pathology
rams
Sperm
spermatozoa
TRIM
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Summary:The present study investigated effects of NOC-18 and TRIM on kinematic parameters of epididymal ram sperm in vitro. After incubation of epididymal sperm samples for 45 and 90 min in the presence of NOC-18 (0.0005, 0.5, and 500 µM) and TRIM (0.1, 10, and 1000 µM), motion parameters were evaluated by computer-aided sperm analysis. There was a significant increase in the fast progressive motility of sperm in the NOC-treated group at a 0.0005 μM. A significant decrease in immotility (at 45 min), BCF (at 45 min), and nonprogressive motility (at 90 min) in the NOC-treated sperm was observed when compared to controls. There was significant increase of the slow progressive (at 45 min) and nonprogressive motility and decrease of the fast progressive motility, VCL, VSL, VAP, ALH, MAD (at 90 min), and STR (at 90 min) of sperm in NOC-treated groups at a concentration of 500 μM as compared to controls. Different concentrations of TRIM appeared significant (p 
ISSN:1618-5641
1618-565X
DOI:10.1007/s00580-009-0911-y