Interleukin-10 protects against lethalit of intra- abdominal infection and sepsis

The aim of this study was to determine whether interleukin-10 would alter locally derived and systemic proinflammatory cytokine expression and protect from the lethality of cecal ligation and puncture. Three groups of Sprague-Dawley rats were used. Group 1 underwent cecal manipulation. Groups 2 and...

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Bibliographic Details
Published in:Journal of gastrointestinal surgery 2000-02, Vol.4 (1), p.70
Main Authors: Rongione, Anthony J, Kusske, Amy M, Kwan, Karen, Ashley, Stanley W, Reber, Howard A, Mcfadden, David W
Format: Article
Language:English
Subjects:
Cytokines
Mortality
Rodents
Sepsis
Online Access:Get full text
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Summary:The aim of this study was to determine whether interleukin-10 would alter locally derived and systemic proinflammatory cytokine expression and protect from the lethality of cecal ligation and puncture. Three groups of Sprague-Dawley rats were used. Group 1 underwent cecal manipulation. Groups 2 and 3 underwent cecal ligation and puncture. Group 2 received intraperitoneal saline injections beginning 1 hour after cecal ligation and puncture and every 3 hours thereafter for 24 hours. Group 3 received intraperitoneal interleukin-10 one hour after cecal ligation and puncture and every 3 hours thereafter. Animals were killed at 6 and 24 hours after cecal ligation and puncture or sham operation. Serum tumor necrosis factor-alpha (TNF-α) levels were determined by enzyme-linked immunosorhent assay. TNF-α messenger RNA expression was determined by reverse transcriptase-polymerase chain reaction using β-actin as the internal standard. There was a twofold increase (P
ISSN:1091-255X
1873-4626
DOI:10.1016/S1091-255X(00)80035-9