Octreotide improves reperfusion-induced oxidative injury in acute abdominal hypertension in rats

• Published in: Journal of gastrointestinal surgery 2004-01, Vol.8 (1), p.113-119
• Main Authors: Kaçmaz, Ayhan, Polat, Ali, User, Yilmaz, Tilki, Metin, Özkan, Sirri, Şener, Göksel
• Format: Article
• Language: English
• Subjects: Abdomen; Abdomen - blood supply; Abdominal hypertension; Animals; Compartment Syndromes - physiopathology; Compartment Syndromes - therapy; Female; Gastrointestinal Agents - pharmacology; Gastrointestinal Agents - therapeutic use; glutathione; Ischemia; lipid peroxidation; Lipid Peroxidation - drug effects; Male; myeloperoxidase; Neutrophil Infiltration - drug effects; octreotide; Octreotide - pharmacology; Octreotide - therapeutic use; Peroxidase - metabolism; Rats; Rats, Wistar; Reperfusion Injury - prevention & control; Reperfusion Injury - therapy; Rodents
• ISSN: 1091-255X; 1873-4626
• DOI: 10.1016/j.gassur.2003.09.026

Ischemia/reperfusion injury plays an important role in the pathogenesis of abdominal compartment syndrome, which is characterized by increased intra-abdominal pressure. The aim of this study was to investigate whether octreotide, a synthetic somatostatin analogue, improves the reperfusion injury after decompression of acute abdominal hypertension. This study was carried out in Wistar albino rats. With the rats under anesthesia, an arterial catheter was inserted intraperioneally and with the use of an aneroid manometer connected to the catheter, intra-abdominal pressure was kept at 20 mm Hg (ischemia group) for 1 hour. In the ischemia/reperfusion group, pressure applied for 1 hour was decompressed and a 1-hour reperfusion period was allowed. In another ischemia/reperfusion group, octreotide was administered (50 μg/kg intraperitoneally) immediately before the decompression of intra-abdominal pressure. At the end of the experiment, liver and intestinal tissues were taken and malondialdehyde (an index of lipid peroxidation) and glutathione (a key to antioxidant) levels and myeloperoxidase (an index of tissue neutrophil infiltration) activity were estimated. The results demonstrated that tissue levels of malondialdehyde and myeloperoxidase activity were elevated, whereas glutathione levels were reduced in both the ischemia and ischemia/reperfusion groups. Octreotide treatment reversed these oxidant responses. In conclusion, increased intra-abdominal pressure causes oxidative organ damage and octreotide, by controlling the reperfusion of abdominal organs and inhibiting neutrophil infiltration, could improve the reperfusion-induced oxidative damage. Therefore its therapeutic role as a “reperfusion injury–limiting” agent must be further elucidated in intra-aortic pressure–induced abdominal organ injury.