Clinical correlations of miR-21 expression in colorectal cancer patients and effects of its inhibition on DLD1 colon cancer cells

Purpose MicroRNA-21 (miR-21) is one of the miRNAs that are frequently and highly overexpressed in tumor tissue of colorectal cancer (CRC) patients; however, only a little is known about its functional role in CRC. Methods We examined the expression level of miR-21 in 44 paired samples of tumoral and...

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Bibliographic Details
Published in:International journal of colorectal disease 2012-11, Vol.27 (11), p.1401-1408
Main Authors: Faltejskova, Petra, Besse, Andrej, Sevcikova, Sabina, Kubiczkova, Lenka, Svoboda, Marek, Smarda, Jan, Kiss, Igor, Vyzula, Rostislav, Slaby, Ondrej
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Antineoplastic Agents - pharmacology
Antineoplastic Agents - therapeutic use
Apoptosis
Apoptosis - drug effects
Apoptosis - genetics
Cancer
Cancer cells
Care and treatment
Cell Cycle - drug effects
Cell Cycle - genetics
Cell Line, Tumor
Cell Movement - drug effects
Cell Movement - genetics
Cell Survival - drug effects
Cell Survival - genetics
Colonic Neoplasms - genetics
Colonic Neoplasms - pathology
Colorectal cancer
Colorectal Neoplasms - drug therapy
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
Female
Gastroenterology
Gene Expression Regulation, Neoplastic - drug effects
Hepatology
Humans
Internal Medicine
Kaplan-Meier Estimate
Male
Medicine
Medicine & Public Health
MicroRNA
MicroRNAs - antagonists & inhibitors
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
Original Article
Prevention
Proctology
Surgery
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Summary:Purpose MicroRNA-21 (miR-21) is one of the miRNAs that are frequently and highly overexpressed in tumor tissue of colorectal cancer (CRC) patients; however, only a little is known about its functional role in CRC. Methods We examined the expression level of miR-21 in 44 paired samples of tumoral and non-tumoral colon tissues diagnosed for CRC using TaqMan real-time PCR method. Furthermore, we used miR-21 inhibitor (anti-miR-21) to transient knockdown of miR-21 in DLD-1 colon cancer cells and examined the effects of miR-21 silencing on viability, apoptosis, chemosensitivity, cell cycle, and migration of DLD1 cells. Results The expression levels of miR-21 were significantly increased in CRC tumor tissue ( P  
ISSN:0179-1958
1432-1262
DOI:10.1007/s00384-012-1461-3