Flavonoids Products from Nitraria retusa Leaves Promote Lymphoblastoid Cells Apoptosis

In this report, the ethyl acetate extract and its major constituent, isorhamnetin 3-o-robinobioside (I3-O-Rob), from Nitraria retusa (N. Retusa) leaf extracts were evaluated for their ability to induce apoptosis in human lymphoblastoid cells (TK6). Apoptosis of TK6 cell line was carried out using DN...

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Bibliographic Details
Published in:Nutrition and cancer 2012-01, Vol.64 (7), p.1095-1102
Main Authors: Boubaker, Jihed, Wissem, Bhouri, Mohammed, Ben Sghaier, Ines, Bouhlel, Mounira, Kriffi, Ines, Skandrani, Genviève, Dijoux Franca Marie, Kamel, Ghedira, Leila, Chekir-Ghedira
Format: Article
Language:English
Subjects:
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Cancer
Caspase 3 - metabolism
caspase-3
Cell Line, Tumor
Cells
Deoxyribonucleic acid
DNA
DNA fragmentation
DNA Fragmentation - drug effects
ethyl acetate
Flavonoids - pharmacology
Flowers & plants
Glycosides - pharmacology
Humans
isorhamnetin
leaf extracts
leaves
Life Sciences
Magnoliopsida - chemistry
NAD ADP-ribosyltransferase
Nitraria
Plant Extracts - pharmacology
Plant Leaves - chemistry
Poly(ADP-ribose) Polymerases - genetics
Poly(ADP-ribose) Polymerases - metabolism
Quercetin - analogs & derivatives
Quercetin - pharmacology
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Summary:In this report, the ethyl acetate extract and its major constituent, isorhamnetin 3-o-robinobioside (I3-O-Rob), from Nitraria retusa (N. Retusa) leaf extracts were evaluated for their ability to induce apoptosis in human lymphoblastoid cells (TK6). Apoptosis of TK6 cell line was carried out using DNA fragmentation, poly ADP-ribose polymerase (PARP) cleavage with reference to caspase-3 activity. These tested products, from N. Retusa, enhanced the apoptosis effects in the tested cancer cell line. This result was confirmed by DNA fragmentation and PARP cleavage indicating a release of caspase-3 level. Our results indicate that the original ethyl acetate extract and the I3-O-Rob have a great antiproliferative effect on human lymphoblastoid cells (TK6), which may be due to their involvement in the apoptotic pathway.
ISSN:1532-7914
0163-5581
1532-7914
DOI:10.1080/01635581.2012.717680