A Method of Creating a Cell Monolayer Based on Organotypic Culture for Screening of Physiologically Active Substances

We developed a method of culturing and phenotyping of a monolayer of cells of the retinal tissue, thymus and spleen on the basis of organotypic culture. All characteristic types of neurons and fi broblasts were found in their microenvironment in the retinal cell monolayer. Lymphocytes, macrophages,...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2012-09, Vol.153 (5), p.795-799
Main Authors: Khavinson, V. Kh, Linkova, N. S., Pronyaeva, V. E., Chalisova, N. I., Koncevaya, E. A., Polyakova, V. O., Kvetnaya, T. V., Kvetnoy, I. M., Yakovlev, G. M.
Format: Article
Language:English
Subjects:
B cells
Biomedical and Life Sciences
Biomedicine
Cell Biology
Internal Medicine
Laboratory Medicine
Methods
Monomolecular films
Pathology
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Summary:We developed a method of culturing and phenotyping of a monolayer of cells of the retinal tissue, thymus and spleen on the basis of organotypic culture. All characteristic types of neurons and fi broblasts were found in their microenvironment in the retinal cell monolayer. Lymphocytes, macrophages, and fi broblasts were verifi ed in the monolayer of thymus and spleen cells. Histological staining, immunocytochemistry, and electron microscopy demonstrated the possibility of assessing the differentiation degree and functional activity of the cell monolayer. The developed technique preserves cell-cell interactions and a variety of cell types characteristic of the examined organ in the monolayer. This opens up new prospects for its application in basic research and in screening of different physiologically active substances.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-012-1829-y