Structure of the Mediator Head module bound to the carboxy-terminal domain of RNA polymerase II

The X-ray crystal structure of the Head module, one-third of the Mediator of transcriptional regulation, has been determined as a complex with the C-terminal domain (CTD) of RNA polymerase II. The structure reveals multiple points of interaction with an extended conformation of the CTD; it suggests...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2012-10, Vol.109 (44), p.17931-17935
Main Authors: Robinson, Philip J. J., Bushnell, David A., Trnka, Michael J., Burlingame, Alma L., Kornberg, Roger D.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Binding Sites
Biochemistry
Biological Sciences
Crystal structure
Crystallography, X-Ray
Crystals
Genes
Genetic mutation
Models, Molecular
Molecular Sequence Data
Molecular structure
Phosphorylation
Protein Conformation
Proteins
RNA
RNA polymerase
RNA Polymerase II - chemistry
RNA Polymerase II - metabolism
Sequence Homology, Amino Acid
Tandem Mass Spectrometry
Transcriptional regulatory elements
Truncation
Yeasts
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Summary:The X-ray crystal structure of the Head module, one-third of the Mediator of transcriptional regulation, has been determined as a complex with the C-terminal domain (CTD) of RNA polymerase II. The structure reveals multiple points of interaction with an extended conformation of the CTD; it suggests a basis for regulation by phosphorylation of the CTD. Biochemical studies show a requirement for Mediator-CTD interaction for transcription.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1215241109