Gene Expression of ACTH, Glucocorticoid Receptors, 11[beta]HSD Enzymes, LH-, FSH-, GH Receptors and Aromatase in Equine Epididymal and Testicular Tissueis

• Published in: Reproduction in domestic animals 2012-12, Vol.47 (6), p.928
• Main Authors: Herrera-Luna, CV, Budik, S, Aurich, C
• Format: Article
• Language: English
• Subjects: Animal reproduction; Gene expression; Horses; Stress response
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/j.1439-0531.2012.01993.x

Contents Glucocorticoids (GCs) are important mediators of the stress response and have been implicated in the function and regulation of testicular functions in different species. In many tissues, intracellular glucocorticoid activity is controlled by either or both of the two known isoforms of 11[beta]-hydroxysteroid dehydrogenase (11[beta]HSD) type 1 and 2, which interconvert active and inactive GCs. Little is known about the effects of stress on fertility in the equine species. The main objective of the present study was to investigate the expression of receptors for GCs and adrenocorticotropic hormone [ACTH, melanocortin 2 receptor (MC2R)] as well 11[beta]HSD1 and 11[beta]HSD2 in male equine epididymal and testicular tissue. In addition, expression of aromatase P-450 and receptors for luteinizing hormone (LHR), follicle stimulating hormone (FSHR) and growth hormone (GHR) was studied. Reverse transcriptase PCR and quantitative real-time PCR were performed in tissue from the epididymis (caput and cauda) and testes collected from nine healthy mature stallions (age 4-10years). mRNA for ACTH and GC receptors as well as 11[beta]HSD1 and -2 were found in epididymal and testicular tissue. Expression of the genes studied was always positive in testicular tissue, while it was inconsistent in epididymal tissue. Quantitative gene expression in relation to [beta]-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was significantly correlated (R=0.403, p