addition of ticarcillin‐clavulanic acid to INRA 96 extender for stallion semen cooling

REASONS FOR PERFORMING STUDY: A commonly used commercial extender (i.e. INRA 96) contains antimicrobials that may have limited effectiveness. Therefore, addition of ticarcillin‐clavulanic acid to this extender is a widespread procedure in the equine breeding industry in the United States. However, s...

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Bibliographic Details
Published in:Equine veterinary journal 2012-12, Vol.44 (S43), p.95-99
Main Authors: Dean, C. J., Hobgood, A. M., Blodgett, G. P., Love, C. C., Blanchard, T. L., Varner, D. D.
Format: Article
Language:English
Subjects:
Acids
antimicrobial
horse
Microbiology
semen extender
Sperm
Statistical methods
viability
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Summary:REASONS FOR PERFORMING STUDY: A commonly used commercial extender (i.e. INRA 96) contains antimicrobials that may have limited effectiveness. Therefore, addition of ticarcillin‐clavulanic acid to this extender is a widespread procedure in the equine breeding industry in the United States. However, such practice has not been critically evaluated. OBJECTIVES: To evaluate the addition of ticarcillin‐clavulanic acid to INRA 96 and different extender and antimicrobial storage conditions on sperm function and antimicrobial effectiveness. METHODS: Gel‐free semen (42 ejaculates from 14 mature Quarter Horse stallions) was extended with INRA 96 and stored for 24 h in an Equitainer II. The effects of added ticarcillin‐clavulanic acid and different extender storage procedures on sperm motion characteristics (by computer‐assisted analysis), sperm membrane integrity (by fluorescence‐based measurement) and suppression of bacterial growth (by aerobic and anaerobic culture methods) were evaluated using analysis‐of‐variance and Chi‐square statistical methods. The P value for significance was set at
ISSN:0425-1644
2042-3306
DOI:10.1111/j.2042-3306.2012.00638.x