Oxidative stress parameters in elderly patients treated for essential hypertension compared with patients treated for type II diabetes and healthy controls

• Published in: Przegląd kardiodiabetologiczny 2011-07, Vol.6 (3), p.234
• Main Authors: Rybka, Joanna, Kedziora-Kornatowska, Kornelia, Kupczyk, Daria, Kozakiewicz, Mariusz, Czuczejko, Jolanta, Szewczyk-Golec, Karolina, Szypula, Jan, Kedziora, Józef, Blaszczak, Robert
• Format: Article
• Language: eng ; pol
• ISSN: 1896-9666; 2084-9877

Aim: To compare values of parameters of oxidative stress in elderly patients treated for essential hypertension (HT), treated for diabetes mellitus type II (DM) and healthy elderly controls (C). Material and methods: The study was carried out in the blood collected from hypertensive and diabetic during their usual medical treatment. The concentration of glutathione (GSH) and activities of catalase (CAT), CuZn superoxide dismutase (CuZn SOD, SOD-1), glutathione peroxidase (GPx-1) and glutathione reductase (GR) as well as the level of malondialdehyde (MDA), F2-isoprostanes and advanced oxidation protein products (AOPP) were measured. Results: We observed increased antioxidant defense in hypertensive patients but not in type 2 diabetes patients when compared with healthy controls. Mean activity of SOD-1 was significantly increased in HT when compared with DM, p < 0.001 (2304.1 ±37.48 U/g Hb and 2130.0 ±35.31 U/g Hb, respectively). Mean activity of CAT was significantly higher in HT group compared with DM p < 0.001 (24.1 ±0.41 BU/g Hb and 21.4 ±0.41 BU/g Hb, respectively). Mean activity of GPx was significantly decreased in DM if compared with C, p < 0.05 (11.8 ±0.45 U/g Hb, 14.3 ±1.00 U/g Hb, respectively). The AOPP concentration was significanty lower in HT (19.7 ±0.73 µmol/l) when comapred with DM (25.3 ±1.32 µmol/l, p < 0.001) and C (30.9 ±3.10 µmol/l, p < 0.001). No differences in GR activity as well as GSH, MDA and F2- isoprostane concentrations between groups were observed. Conclusions: We did not observe any straightforward indication of oxidative stress neither in hypertensives nor in diabetics when compared with age and sex matched controls. Upregulation of antioxidant defense in hypertension group observed in our study was most likely caused by antihypertensive treatment. Altogether our results suggest that hypertensive patients treated for their conditions might be better protected from cardiovascular events related to oxidative stress than type 2 diabetes patients on hypoglycemic treatment.