Toxicity of Functionalized Single-Walled Carbon Nanotubes on Soil Microbial Communities: Implications for Nutrient Cycling in Soil

• Published in: Environmental science & technology 2013-01, Vol.47 (1), p.625-633
• Main Authors: Rodrigues, Debora F, Jaisi, Deb P, Elimelech, Menachem
• Format: Article
• Language: English
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Applied ecology; Bacteria - drug effects; Bacteria - genetics; Bacteria - metabolism; Bacterial Load; Biogeochemistry; Biological and medical sciences; Carbon; Cloning; Ecotoxicology, biological effects of pollution; Fundamental and applied biological sciences. Psychology; Fungi - drug effects; Fungi - genetics; General aspects; Genes, Bacterial; Genes, Fungal; Microbial ecology; Microbiology; Nanotubes; Nanotubes, Carbon - toxicity; Phosphorus; Physiology; Polymorphism; Soil Microbiology; Soil Pollutants - toxicity; Synecology; Various environments (extraatmospheric space, air, water)
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es304002q

Culture-dependent and -independent methods were employed to determine the impact of carboxyl-functionalized single-walled carbon nanotubes (SWNTs) on fungal and bacterial soil microbial communities. Soil samples were exposed to 0 (control), 250, and 500 μg of SWNTs per gram of soil. Aliquots of soil were sampled for up to 14 days for culture-dependent analyses, namely, plate count agar and bacterial community level physiological profiles, and culture-independent analyses, namely, quantitative real-time polymerase chain reaction (qPCR), mutliplex-terminal restriction fragment length polymorphism (M-TRFLP), and clone libraries. Results from culture-independent and -dependent methods show that the bacterial soil community is transiently affected by the presence of SWNTs. The major impact of SWNTs on bacterial community was observed after 3 days of exposure, but the bacterial community completely recovered after 14 days. However, no recovery of the fungal community was observed for the duration of the experiment. Physiological and DNA microbial community analyses suggest that fungi and bacteria involved in carbon and phosphorus biogeochemical cycles can be adversely affected by the presence of SWNTs. This study suggests that high concentrations of SWNTs can have widely varying effects on microbial communities and biogeochemical cycling of nutrients in soils.