Relationship of serum paraoxonase enzyme activity and thermal burn injury/Termal yanik ile serum paraoksonaz enzim aktiviteleri arasindaki iliski

• Published in: The Eurasian journal of medicine 2012-12, Vol.44 (3), p.153
• Main Authors: Yildirim, Serap, Doganay, Songul, Yildirim, Abdulkadir, Aydin, Osman Enver, Karakoc, Akar, Laloglu, Esra
• Format: Article
• Language: English
• Subjects: Antioxidants; Biological markers; Blood lipoproteins; Burns; Burns and scalds; Care and treatment; Free radicals; Lipids; Lipoproteins; Oxidative stress; Physiological aspects; Proteolipids; Rodents
• ISSN: 1308-8734; 1308-8742
• DOI: 10.5152/eajm.2012.36

Objective: This study investigated changes in serum oxidative stress parameters in burn cases compared to healthy controls. Materials and Methods: This study was performed in 41 burn patients with mild to severe thermal burn injuries and 38 healthy volunteers. The burn cases were selected from patients who were hospitalized in the burn unit for the treatment of second- and third-degree burns. Malondialdehyde (MDA) levels and PON-1 paraoxonase and arylesterase activities were measured in patient serum samples. Results: PON-1 paraoxonase activity and MDA levels in patients with major thermal burn injury were significantly higher than healthy controls, but PON-1 arylesterase activities were lower. A significant negative correlation was observed between the burn percentage of the total body surface area and the PON-1 arylesterase activities in patients. Conclusion: Human thermal burn injury was associated with an increase in MDA production and a decrease in PON-1 arylesterase activity, which was proportional to the percentage of total burned surface area. Key Words: Arylesterase, Malondialdehyde, Oxidative stress, Paraoxonase, Thermal burn Amac: Bu calismada yanik vakalarinda serum oksidatif stres parametrelerinde meydana gelen muhtemel degisikliklerin arastirilmasi ve saglikli kisilerle karsilastirilmasi amaclandi. Gerec ve Yontem: Calisma hafiften siddetliye 41 termal yanikli hasta ve 38 saglikli gonullu uzerinde yurutuldu. Vakalar tedavi amacli yanik unitesine yatirilan 2. ve 3. derece yanigi olan hastalar arasindan secildi. Malondialdehit (MDA) duzeyleri ve PON-1 paraoksonaz ve arilesteraz aktiviteleri deneklerden alinan kan orneklerinde olculdu. Bulgular: Saglikli kontrollerle karsilastirildiginda PON-1 paraoksonaz aktivitesi ve MDA seviyesi major termal yanikli hastalarda anlamli duzeyde daha yuksek, PON-1 arilesteraz aktivitesi ise daha dusuktu. Ayrica hastalarda PON-1 arilesteraz aktivitesi ile vucut yanik yuzey alani yuzdesi arasinda negatif bir korelasyon vardi. Sonuc: Insanlarda termal yanik, artmis MDA olusumu ve azalmis PON-1 arilesteraz aktivitesi ile iliskilidir. Ayni zamanda azalmis PON-1 arilesteraz aktivitesi yanik yuzey alaninin buyuklugu ile orantili olarak degismektedir. Anahtar Kelimeler: Arilesteraz, Malondialdehit, Oksidatif stress, Paraoksonaz, Termal yanik