LDHA is necessary for the tumorigenicity of esophageal squamous cell carcinoma

Esophageal squamous cell carcinoma (ESCC) is one of the most common lethal tumors in the world, and the development of new therapeutic targets is needed. Recent studies have shown that aerobic glycolysis, also known as the Warburg effect, mediated the anti-apoptotic effects in cancer cells. Lactate...

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Bibliographic Details
Published in:Tumor biology 2013-02, Vol.34 (1), p.25-31
Main Authors: Yao, Feng, Zhao, Tiejun, Zhong, Chenxi, Zhu, Ji, Zhao, Heng
Format: Article
Language:English
Subjects:
Adult
Apoptosis - genetics
Biomedical and Life Sciences
Biomedicine
Cancer
Cancer Research
Carcinoma, Squamous Cell - enzymology
Carcinoma, Squamous Cell - pathology
Caspase 8 - metabolism
Cell Cycle - genetics
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cell Transformation, Neoplastic - genetics
Cellular biology
Cyclin D1 - biosynthesis
Enzyme Activation
Esophageal Neoplasms - enzymology
Esophageal Neoplasms - pathology
Esophageal Squamous Cell Carcinoma
Esophagus
Female
Glycolysis
Humans
Isoenzymes - genetics
Isoenzymes - metabolism
L-Lactate Dehydrogenase - genetics
L-Lactate Dehydrogenase - metabolism
Male
Middle Aged
Pathogenesis
Poly(ADP-ribose) Polymerases - metabolism
Proteins
Proto-Oncogene Proteins c-akt - biosynthesis
Proto-Oncogene Proteins c-akt - metabolism
Research Article
RNA Interference
RNA, Small Interfering
Signal Transduction
Up-Regulation
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Summary:Esophageal squamous cell carcinoma (ESCC) is one of the most common lethal tumors in the world, and the development of new therapeutic targets is needed. Recent studies have shown that aerobic glycolysis, also known as the Warburg effect, mediated the anti-apoptotic effects in cancer cells. Lactate dehydrogenase A (LDHA) which executed the final step of aerobic lactate production has been reported to be involved in the tumor progression. However, the function of LDHA in ESCC has not been investigated. In this study, it was found that LDHA was up-regulated in ESCC clinical samples. Knockdown of the expression of LDHA inhibited cell growth and cell migration in vitro as well as tumorigenesis in vivo. With regard to the molecular mechanism, silencing the expression of LDHA was related to decreased AKT activation and cyclin D1 expression and increased cleavage of PARP and caspase 8. Taken together, our findings suggest that LDHA plays an important role in the progression of ESCC by modulating cell growth, and LDHA might be a potential therapeutic target in ESCC.
ISSN:1010-4283
1423-0380
DOI:10.1007/s13277-012-0506-0