Dye sorption as an indicator of erythrocyte membrane damage and prehemolytic state of erythrocytes

High erythrocyte membrane permeability caused by detergents was associated with increased methylene blue sorption, which correlated with erythrocyte damage and preceded hemolysis. The content of sorbed dye in erythrocytes preincubated with 200 μM sodium dodecyl sulfate or cetyltrimethylammonium brom...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2000-03, Vol.129 (3), p.306-308
Main Authors: Gavrilov, V. B., Kravchenko, O. N., Konev, S. V.
Format: Article
Language:English
Subjects:
Erythrocytes
Sorption
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Summary:High erythrocyte membrane permeability caused by detergents was associated with increased methylene blue sorption, which correlated with erythrocyte damage and preceded hemolysis. The content of sorbed dye in erythrocytes preincubated with 200 μM sodium dodecyl sulfate or cetyltrimethylammonium bromide increased by 58-74% and 2.4-3.8-fold surpassed that in the medium. New precise and convenient method for estimating erythrocyte binding capacity is proposed.[PUBLICATION ABSTRACT]
ISSN:0007-4888
1573-8221
DOI:10.1007/BF02433915