Abscisic acid is required for somatic embryo initiation through mediating spatial auxin response in Arabidopsis

Abscisic acid (ABA) regulates many aspects of plant development, including somatic embryo (SE) initiation. However, mechanisms of ABA functions on SE initiation have remained to be investigated. In this study, we examined the endogenous ABA contents of calli in Arabidopsis during the SE inductive pr...

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Bibliographic Details
Published in:Plant growth regulation 2013-03, Vol.69 (2), p.167-176
Main Authors: Su, Ying Hua, Su, Yu Xiao, Liu, Ying Gao, Zhang, Xian Sheng
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Biosynthesis
Embryos
Life Sciences
Original Paper
Plant Anatomy/Development
Plant Physiology
Plant Sciences
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Summary:Abscisic acid (ABA) regulates many aspects of plant development, including somatic embryo (SE) initiation. However, mechanisms of ABA functions on SE initiation have remained to be investigated. In this study, we examined the endogenous ABA contents of calli in Arabidopsis during the SE inductive process. We further found that the capacity for SE initiation was strongly impaired by treatment of fluridone, a potent inhibitor of ABA biosynthesis, as well as by mutation of ABA biosynthetic gene ABA2 , suggesting that ABA is required for SE initiation. Furthermore, treatment of fluridone inhibited local auxin biosynthesis and auxin polar transport in the embryonic calli, resulting in the disturbance of auxin response pattern and the decreased regeneration frequency of SEs. However, application of exogenous ABA in the medium almost recovered patterns of auxin response and SE initiation. Thus, the results suggest that ABA functions on SE initiation through mediating both auxin biosynthesis and polar transport for establishment of auxin response pattern in callus. Our study provides new information for understanding mechanisms of SE initiation.
ISSN:0167-6903
1573-5087
DOI:10.1007/s10725-012-9759-2