A Spot Test for Estimating Ureides in Soyabean Petioles

The ureides allantoic acid and allantoin form a bright pink compound with acidic p-dimethylamino cinnamaldehyde. It is possible to estimate the ureide content of a soyabean petiole segment by crushing it on filter paper and developing the colour with this reagent. There is a strong correlation betwe...

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Bibliographic Details
Published in:Experimental agriculture 1986-10, Vol.22 (4), p.405-416
Main Authors: Glenister, Richard A., LaRue, Thomas A.
Format: Article
Language:English
Subjects:
acetylene
Agronomy. Soil science and plant productions
allantoic acid
allantoin
Biological and medical sciences
Chemical constitution
Economic plant physiology
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Glycine max
nitrogen fixation
petiole analysis
reduction
Soil-plant relationships. Soil fertility. Fertilization. Amendments
spot tests
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Summary:The ureides allantoic acid and allantoin form a bright pink compound with acidic p-dimethylamino cinnamaldehyde. It is possible to estimate the ureide content of a soyabean petiole segment by crushing it on filter paper and developing the colour with this reagent. There is a strong correlation between the spot test and estimates from colorimetric determination of ureides. In field grown soyabeans, there was a positive correlation between the petiole ureide content during pod fill and the integrated seasonal acetylene reduction activity. The spot test can be used to identify plants with low nitrogen fixation activity.
ISSN:0014-4797
1469-4441
DOI:10.1017/S0014479700014654