An Improved Method for the Histological Preparation of Single Hairs and Dust Samples-Morphological and Immunological Examination

An improved method of the serological and morphological investigation of human hair is reported. The hair was firmly fixed onto a microscopic slide with cellophane tape and observed microscopically to confirm the cuticula images and the presence of the medulla. A piece of the hair containing the med...

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Bibliographic Details
Published in:Journal of forensic sciences 1988-05, Vol.33 (3), p.767-772
Main Authors: Tanabe, R, Ishiyama, I, Itakura, Y
Format: Article
Language:English
Subjects:
Adhesives
Blood Grouping and Crossmatching - methods
Dust
Forensic sciences
Hair - analysis
Hair - anatomy & histology
Histological Techniques
Humans
Immunoenzyme Techniques
Male
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Summary:An improved method of the serological and morphological investigation of human hair is reported. The hair was firmly fixed onto a microscopic slide with cellophane tape and observed microscopically to confirm the cuticula images and the presence of the medulla. A piece of the hair containing the medulla was dissected, embedded in paraffin, and a cross section of this hair was prepared. By treating the sample with immunohistochemistry (biotin-antibiotin ABC technique), the blood type of the hair was confirmed definitively. Dust containing shaved beard can be examined in the same way.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS12487J