Glyoxalase I Typing and Phosphoglucomutase-1 Subtyping of a Single Hair

A technique is described for the typing of glyoxalase I (GLO I) and the subtyping of phosphoglucomutase-1 (PGM-1) from the root sheath cells of a single forcibly removed hair. This procedure does not require sample preparation and does not alter the morphological characteristics of the hair. The com...

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Bibliographic Details
Published in:Journal of forensic sciences 1987-09, Vol.32 (5), p.1175-1181
Main Authors: Gambel, AM, Budowle, B, Terrell, L
Format: Article
Language:English
Subjects:
Forensic Medicine - methods
Genetic Markers
Hair - enzymology
Hair analysis
Humans
Isoelectric Focusing
Lactoylglutathione Lyase - analysis
Lyases - analysis
Phosphoglucomutase - analysis
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Summary:A technique is described for the typing of glyoxalase I (GLO I) and the subtyping of phosphoglucomutase-1 (PGM-1) from the root sheath cells of a single forcibly removed hair. This procedure does not require sample preparation and does not alter the morphological characteristics of the hair. The combined discrimination probability (DP) of the two markers taken together is 0.90 for whites and 0.89 for blacks. GLO I can be typed after four weeks, and PGM-1 can be typed after eight to fifteen weeks in hairs maintained at room temperature. Hairs mounted with Permount® showed loss of enzyme activity and loss of band sharpness.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS11169J