A Microplate Method for Reverse ABO Typing of Bloodstains

A sensitive and reliable hemagglutination assay, using V-bottom microplates, is described for the detection of the ABO blood group alloantibodies in bloodstained material. When used in conjunction with an absorption-elution procedure, the microplate assay resulted in a 300% increase in the number of...

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Bibliographic Details
Published in:Journal of forensic sciences 1986-04, Vol.31 (2), p.418-425
Main Author: Mudd, JL
Format: Article
Language:English
Subjects:
ABO Blood-Group System - genetics
Blood Grouping and Crossmatching - methods
Blood Preservation - methods
Blood Stains
Evidence
Forensic hematology
Hemagglutination Tests - methods
Humans
Isoantibodies - genetics
Phenotype
Temperature
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Summary:A sensitive and reliable hemagglutination assay, using V-bottom microplates, is described for the detection of the ABO blood group alloantibodies in bloodstained material. When used in conjunction with an absorption-elution procedure, the microplate assay resulted in a 300% increase in the number of conclusive grouping results when compared to the Lattes crust test. The use of the microplate reverse grouping assay permits 24 specimens to be assayed conveniently on a single plate and eliminates the tedious and time-consuming microscopic examination required for the Lattes crust test.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS12271J