Cultivating oral mucosa epithelial transplantation (COMET) followed by penetrating keratoplasty (PK) in ocular surface reconstruction

Purpose Efficacy of COMET and subsequent PK in patients with limbal insufficiency combined with stromal corneal haze after corneal burns Methods 15 patients (16 eyes)who underwent corneal burns with vascular conjuctival pannus and stromal involvement underwent autologous cultivated oral mucosa epith...

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Published in:Acta ophthalmologica (Oxford, England) England), 2012-09, Vol.90 (s249), p.0-0
Main Authors: DOBROWOLSKI, D, WOWRA, B, ORZECHOWSKA‐WYLEGALA, B, WYLEGALA, E
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Language:English
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Ophthalmology
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WOWRA, B
ORZECHOWSKA‐WYLEGALA, B
WYLEGALA, E
description Purpose Efficacy of COMET and subsequent PK in patients with limbal insufficiency combined with stromal corneal haze after corneal burns Methods 15 patients (16 eyes)who underwent corneal burns with vascular conjuctival pannus and stromal involvement underwent autologous cultivated oral mucosa epithelial transplantation. Cellular material was obtained from buccal mucosa, tripsinized, and settled on the previously prepared culture dishes. Cultures was carried on denuded amniotic membrane in presence of inactivated with mitomycin C 3T3 fibroblasts. At least 12 month after epithelium restoration patients underwent PK. Corneal surface stability and visual acuity were evaluated. Results 6 month after last step of the surgery 68,7% of eyes had transparent cornea without reccurent revascularization, 31,2 % of eyes developed vascular pannus again with vascular ingrowth on the transplanted cornea ( in 2 cases visual axis was involved), in 62,5 % of eyes visual acuity increased from mean 0,01 to 0,3. Conclusion COMET combined with PK effectively improves ocular surface stability and quality of vision.
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Cellular material was obtained from buccal mucosa, tripsinized, and settled on the previously prepared culture dishes. Cultures was carried on denuded amniotic membrane in presence of inactivated with mitomycin C 3T3 fibroblasts. At least 12 month after epithelium restoration patients underwent PK. Corneal surface stability and visual acuity were evaluated. Results 6 month after last step of the surgery 68,7% of eyes had transparent cornea without reccurent revascularization, 31,2 % of eyes developed vascular pannus again with vascular ingrowth on the transplanted cornea ( in 2 cases visual axis was involved), in 62,5 % of eyes visual acuity increased from mean 0,01 to 0,3. 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title Cultivating oral mucosa epithelial transplantation (COMET) followed by penetrating keratoplasty (PK) in ocular surface reconstruction
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