Antioxidant Activity of Flavonoids of Different Polarity, Assayed by Modified ABTS Cation Radical Decolorization and EPR Technique

Modified ABTS cation radical decolorization assay and EPR technique were applied to screen the antioxidant activity of three flavonoids with different polarity: 7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine), 4'-methoxy-5,7-dihydroxyflavone 6-C-β-glucopyranoside (...

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Published in:Acta biologica cracoviensia. Series Botanica. 2010-01, Vol.52 (1), p.97-104
Main Authors: Pawlak, Krzysztof, Bylka, Wieslawa, Jazurek, Beata, Matlawska, Irena, Sikorska, Maria, Manikowski, Henryk, Bialek-Bylka, Grazyna
Format: Article
Language:English
Subjects:
4'-methoxy-5
7-dihydroxyflavone 6-C-β-glucopyranoside (isocytisoside)
7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine)
ABTS cation radical
all-trans β-carotene
EPR technique
free radicals
I 3' II 8 biapigenine (amentoflavone)
oxidation
TEAC
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cited_by cdi_FETCH-LOGICAL-c354t-d8d106cb994d2e1401059942b67232b6fbc755f81de82faabcbd42ecbc2b86803
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container_title Acta biologica cracoviensia. Series Botanica.
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creator Pawlak, Krzysztof
Bylka, Wieslawa
Jazurek, Beata
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Sikorska, Maria
Manikowski, Henryk
Bialek-Bylka, Grazyna
description Modified ABTS cation radical decolorization assay and EPR technique were applied to screen the antioxidant activity of three flavonoids with different polarity: 7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine), 4'-methoxy-5,7-dihydroxyflavone 6-C-β-glucopyranoside (isocytisoside) and I 3' II 8 biapigenine (amentoflavone), with nonpolar all-trans β-carotene used as standard carotenoid molecule. The ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid] cation radical decolorization assay was modified as follows: (1) measurements extended up to 8 days after preparation, (2) method adapted for flavonoids with different polarity and β-carotene, (3) concentrations in the 0.01-10 μM range of both trolox and antioxidants in order to use the same experimental conditions for both this technique and EPR measurement.
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subjects 4'-methoxy-5
7-dihydroxyflavone 6-C-β-glucopyranoside (isocytisoside)
7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine)
ABTS cation radical
all-trans β-carotene
EPR technique
free radicals
I 3' II 8 biapigenine (amentoflavone)
oxidation
TEAC
title Antioxidant Activity of Flavonoids of Different Polarity, Assayed by Modified ABTS Cation Radical Decolorization and EPR Technique
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