Comparison between two cyclooxygenase inhibitors in an experimental dry eye model in albino rabbits

• Published in: Acta pharmaceutica (Zagreb, Croatia) Croatia), 2008-06, Vol.58 (2), p.163-173
• Main Authors: EL-Shazly, Amany, EL-Gohary, Amal, EL-Shazly, Laila, EL-Hossary, Ghada
• Format: Article
• Language: English
• Subjects: Administration, Topical; Animals; atropin sulfat; Atropine; atropine sulphate; Cyclooxygenase 2 - metabolism; Cyclooxygenase 2 Inhibitors - administration & dosage; Cyclooxygenase 2 Inhibitors - pharmacology; Cyclooxygenase Inhibitors - administration & dosage; Cyclooxygenase Inhibitors - pharmacology; Disease Models, Animal; dry eye model; Dry Eye Syndromes - chemically induced; Dry Eye Syndromes - drug therapy; Dry Eye Syndromes - enzymology; Dry Eye Syndromes - pathology; Eye - drug effects; Eye - enzymology; Eye - pathology; ketorolac; Ketorolac - administration & dosage; Ketorolac - pharmacology; ketorolak; kunić; Male; model suhog oka; nimesulid; nimesulide; Ophthalmic Solutions; Prostaglandin-Endoperoxide Synthases - metabolism; rabbit; Rabbits; Schirmer test I; Schirmerov test I; Sulfonamides - administration & dosage; Sulfonamides - pharmacology; Tears - metabolism; Time Factors
• ISSN: 1330-0075; 1846-9558
• DOI: 10.2478/v10007-008-0009-0

The purpose of this study was to compare the topical anti-inflammatory effects of the nonselective cyclooxygenase (COX) inhibitor, ketorolac, with the selective COX-2 inhibitor, nimesulide, in an animal model of dry eye in albino rabbits. All animals were examined by the Schirmer test, tear break-up time (TBUT) and fluorescein corneal staining test. Dry eye model showed significant reduction in tear volume, TBUT, corneal staining and histopathological signs of dryness and inflammation. On treating dry eye model with nimesulide 0.1% eye drops and ketorolac 0.5% eye drops, there were improvements in Schirmer test values, TBUT and fluorescein corneal staining and histopathologically reduced inflammatory reaction, with signs of healing and regeneration. Both nimesulide and ketorolac ameliorate atropine sulphate induced dry eye in albino rabbits. The use of selective COX-2 inhibitor, nimesulide, is preferred to avoid local and systemic side effects which may occur with the use of the nonselective COX inhibitor, ketorolac. Cilj istraživanja bila je usporedba topičkog protuupalnog učinka neselektivnog inhibitora ciklooksigenaze (COX), ketorolaka i selektivnog COX-2 inhibitora, nimesulida na animalnom modelu suhog oka u albino kunića. Na životinjama je proveden Schirmerov test, vrijeme prestanka suzenja oka (TBUT) i test bojenja rožnice fluoresceinom. U animalnom modelu suhog oka značajno je smanjen volumen suza, TBUT, bojenje rožnice i histopatološki simptomi suhoće i upale. Obradom suhog oka s kapima za oči koje sadrže 0,1% nimesulida, odnosno 0,5% ketorolaka, poboljšane su vrijednosti u Schirmerovom testu, TBUT i bojanje rožnice fluoresceinom, smanjena je upalna reakcija, a na oku su se pokazali simptomi ozdravljenja i regeneracije. I nimesulid i ketorolak smanjuju suhoću oka induciranu atropin sulfatom u albino kunića. Uporaba selektivnog COX-2 inhibitora, nimesulida, se preferira jer se izbjegavaju lokalne i sistemske nuspojave koje se mogu pojaviti uz uporabu neselektivnog COX inhibitora, ketorolaka.