Phenol red inhibits chondrogenic differentiation and affects osteogenic differentiation of human mesenchymal stem cells in vitro

The purpose with this study was to investigate the effect of phenol red (PR) on chondrogenic and osteogenic differentiation of human mesenchymal stem cells (hMSCs). hMSCs were differentiated into chondrogenic and osteogenic directions in DMEM with and without PR for 2, 7, 14, 21, and 28 days. Gene e...

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Bibliographic Details
Published in:Stem cell reviews and reports 2013-04, Vol.9 (2), p.132
Main Authors: Lysdahl, Helle, Baatrup, Anette, Nielsen, Anna Bay, Foldager, Casper Bindzus, Bünger, Cody
Format: Article
Language:English
Subjects:
Aggrecans - antagonists & inhibitors
Aggrecans - genetics
Aggrecans - metabolism
Alkaline Phosphatase - metabolism
Calcium - metabolism
Cell Differentiation
Cells, Cultured
Chondrocytes - cytology
Chondrocytes - drug effects
Chondrocytes - metabolism
Collagen Type II - antagonists & inhibitors
Collagen Type II - genetics
Collagen Type II - metabolism
Collagen Type X - antagonists & inhibitors
Collagen Type X - genetics
Collagen Type X - metabolism
Culture Media - chemistry
Culture Media - pharmacology
Gene Expression - drug effects
Humans
Male
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - drug effects
Mesenchymal Stem Cells - metabolism
Osteocytes - cytology
Osteocytes - drug effects
Osteocytes - metabolism
Phenolsulfonphthalein - pharmacology
Proteoglycans - biosynthesis
SOX9 Transcription Factor - antagonists & inhibitors
SOX9 Transcription Factor - genetics
SOX9 Transcription Factor - metabolism
Young Adult
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Summary:The purpose with this study was to investigate the effect of phenol red (PR) on chondrogenic and osteogenic differentiation of human mesenchymal stem cells (hMSCs). hMSCs were differentiated into chondrogenic and osteogenic directions in DMEM with and without PR for 2, 7, 14, 21, and 28 days. Gene expression of chondrogenic and osteogenic markers were analyzed by RT-qPCR. The presence of proteoglycans was visualized histologically. Osteogenic matrix deposition and mineralization were examined measuring the alkaline phophatase activity and calcium deposition. During chondrogenic differentiation PR decreased sox9, collagen type 2, aggrecan on day 14 and 21 (P 
ISSN:2629-3269
2629-3277
DOI:10.1007/s12015-012-9417-0