5-Hydroxytryptamine-induced bladder hyperactivity via the 5-HT^sub 2A^ receptor in partial bladder outlet obstruction in rats

We investigated the effects of partial bladder outlet obstruction (BOO) on the function and gene expression of 5-hydroxytryptamine (5-HT) receptor subtypes in rat bladder. Isometric contractions of the isolated bladders from sham-operated control and BOO rats were examined. The contractile responses...

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Bibliographic Details
Published in:American journal of physiology. Renal physiology 2013-04, Vol.304 (7), p.F1020
Main Authors: Sakai, Takumi, Kasahara, Ken-ichi, Tomita, Ken-ichi, Ikegaki, Ichiro, Kuriyama, Hiroshi
Format: Article
Language:English
Subjects:
Bladder
Gene expression
Kidney diseases
Polymerase chain reaction
Ribonucleic acid
RNA
Rodents
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Summary:We investigated the effects of partial bladder outlet obstruction (BOO) on the function and gene expression of 5-hydroxytryptamine (5-HT) receptor subtypes in rat bladder. Isometric contractions of the isolated bladders from sham-operated control and BOO rats were examined. The contractile responses to 5-HT were significantly increased in BOO rat bladder strips, while the responses to KCl, carbachol, or phenylephrine were not different from the control. The 5-HT-induced hypercontraction in BOO rat bladder strips was inhibited by ketanserin, a 5-HT2A receptor antagonist. The contractile responses to 5-HT in bladder strips were not affected by urothelium removal from the intact bladder. The gene expression of 5-HT receptor subtypes in the bladders was analyzed by RT-PCR. The mRNA expression of the 5-HT2A, 5-HT2B, 5-HT2C, 5-HT4, and 5-HT7 receptors was detected in both the control and BOO rat bladders. Quantitative RT-PCR analysis showed there was a significant increase of 5-HT2A receptor mRNA in the BOO rat bladder compared with the control bladder. On the other hand, the gene expression of the 5-HT4 receptor was not changed in the BOO rat bladder. These results suggest that the increased contractile responses to 5-HT in BOO rat bladder may be partly caused by 5-HT2A receptor upregulation in the detrusor smooth muscles. [PUBLICATION ABSTRACT]
ISSN:1931-857X
1522-1466