Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer using [gamma]-H2AX foci of DNA damage in lymphocytes

The purpose of our study was to evaluate the degree of radiotoxicity to lymphocytes in thyroid cancer after iodine-131(I-131) therapy using [gamma]-H2AX foci immunodetection. This study focused on 15 patients who underwent I-131 therapy for differentiated thyroid cancer after surgery. All patients r...

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Bibliographic Details
Published in:Annals of nuclear medicine 2013-04, Vol.27 (3), p.233
Main Authors: Doai, Mariko, Watanabe, Naoto, Takahashi, Tomoko, Taniguchi, Mitsuru, Tonami, Hisao, Iwabuchi, Kuniyoshi, Kayano, Daiki, Fukuoka, Makoto, Kinuya, Seigo
Format: Article
Language:English
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Summary:The purpose of our study was to evaluate the degree of radiotoxicity to lymphocytes in thyroid cancer after iodine-131(I-131) therapy using [gamma]-H2AX foci immunodetection. This study focused on 15 patients who underwent I-131 therapy for differentiated thyroid cancer after surgery. All patients received 3.7 GBq of I-131. Venous blood samples were collected from each patient before therapy and 4 days thereafter. Lymphocytes were isolated from the blood samples and subjected to [gamma]-H2AX immunofluorescence staining. The number (mean ±Â SD) of foci per lymphocyte nucleus was 0.41 ±Â 0.51 before and 6.19 ±Â 1.80 after radioiodine therapy, and this difference was statistically significant (P = 0.001Â
ISSN:0914-7187
1864-6433
DOI:10.1007/s12149-012-0678-0