Capillary electrophoretic and extraction conditions for the analysis of Catha edulisFORKS active principles

A capillary electrophoretic method, which allowed the detection and separation of the active principles of Catha edulis, i.e. cathinone, cathine and phenylpropanolamine, was developed. A suitable internal standard (nicotinamide), which permitted the quantification of the analytes reducing the variab...

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Bibliographic Details
Published in:Forensic science international 2013-05, Vol.228 (1-3), p.154-159
Main Authors: Roda, Gabriella, Liberti, Vincenzo, Arnoldi, Sebastiano, Argo, Antonella, Rusconi, Chiara, Suardi, Sonia, Gambaro, Veniero
Format: Article
Language:English
Subjects:
Catha edulis
Cathine
Cathinone
Forensic sciences
Leaves
Phenylpropanolamine
Principles
Online Access:Get full text
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Summary:A capillary electrophoretic method, which allowed the detection and separation of the active principles of Catha edulis, i.e. cathinone, cathine and phenylpropanolamine, was developed. A suitable internal standard (nicotinamide), which permitted the quantification of the analytes reducing the variability of the migration times due to EOF changes, was identified. The analytical method was validated, assessing linearity, sensitivity and repeatability, showing optimal features for the analysis of the vegetable material. Moreover extraction conditions were investigated to achieve the exhaustion of the plant material in the fastest and most efficient way to meet the requirements of the Court.
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2013.02.034