Spray-Dried Chitosan Microparticles for Cellular Delivery of an Antigenic Protein: Physico-chemical Properties and Cellular Uptake by Dendritic Cells and Macrophages

• Published in: Pharmaceutical research 2013-06, Vol.30 (6), p.1677-1697
• Main Authors: Kusonwiriyawong, Chirasak, Lipipun, Vimolmas, Vardhanabhuti, Nontima, Zhang, Qiang, Ritthidej, Garnpimol C.
• Format: Article
• Language: English
• Subjects: Animals; Antigens; Antigens - administration & dosage; Antigens - chemistry; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Cell Survival - drug effects; Cells, Cultured; Cellular biology; Chitosan - chemistry; Dendritic Cells - metabolism; Drug delivery systems; Drug Delivery Systems - methods; General pharmacology; Hydrogen-Ion Concentration; Lactic Acid - chemistry; Macrophages - metabolism; Medical Law; Medical sciences; Mice; Microspheres; Nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Particle Size; Pharmaceutical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Pharmacology/Toxicology; Pharmacy; Polyglycolic Acid - chemistry; Polymers - administration & dosage; Polymers - chemistry; Proteins; Proteins - administration & dosage; Proteins - chemistry; Research Paper; Serum Albumin, Bovine - administration & dosage; Serum Albumin, Bovine - chemistry
• ISSN: 0724-8741; 1573-904X
• DOI: 10.1007/s11095-013-1014-7

ABSTRACT Purpose Spray-dried chitosan microparticles for cellular delivery of antigen to dendritic cells (DC) and macrophages (Mϕ) were investigated. Methods Chitosan microparticles were prepared by spray drying. For comparison, poly(lactic-co-glycolic acid) (PLGA) and poly(α-butyl cyanoacrylate) (BCA) micro-/nanoparticles were generated. Bovine serum albumin (BSA) was used as a model antigen. The particles were characterized in terms of size, morphology, surface charge, surface composition, protein content, entrapment efficiency, in vitro release, and protein integrity. Additionally, they were subject to cell viability and cellular uptake study with DC and Mϕ. Results Size of chitosan, PLGA, and BCA micro-/nanoparticles ranged between 3.11–7.18, 0.94–6.26, and 0.30–6.34 μm, respectively. Particle morphology and in vitro protein release varied, depending on polymer type, particle composition and preparation process parameters. Chitosan microparticles were cationic, while PLGA microparticles were neutral. BCA micro-/nanoparticles were either anionic or cationic, according to polymerization pH. Protein content and entrapment efficiency of chitosan and PLGA microparticles were relatively consistent. Only integrity and conformational structure of protein encapsulated in chitosan microparticles were completely retained. Chitosan and PLGA microparticles were non-toxic to DC and Mϕ, but the former were internalized more efficiently. Conclusions Spray-dried chitosan microparticles delivered the antigen efficiently to DC and Mϕ.