Proteomic and physiological analyses reveal detoxification and antioxidation induced by Cd stress in Kandelia candel roots

• Published in: Trees (Berlin, West) West), 2013-06, Vol.27 (3), p.583-595
• Main Authors: Weng, Zhao-Xia, Wang, Ling-Xia, Tan, Fang-lin, Huang, Li, Xing, Jian-Hong, Chen, Shi-Pin, Cheng, Chi-Lien, Chen, Wei
• Format: Article
• Language: English
• Subjects: adenosine triphosphate; Agriculture; Biomedical and Life Sciences; Biosynthesis; Cadmium; Detoxification; enzymes; Estuaries; Forestry; gene expression regulation; Heavy metals; Life Sciences; NAD (coenzyme); NADP (coenzyme); Original Paper; Oxidative stress; Phytoremediation; Plant Anatomy/Development; Plant Pathology; Plant Physiology; Plant Sciences; pollutants; prediction; protein synthesis; Proteins; proteomics; Roots
• ISSN: 0931-1890; 1432-2285
• DOI: 10.1007/s00468-012-0811-7

The heavy metal Cadmium (Cd), added to the water bodies through weathering of rocks and human activities, constitutes one of the major environmental pollutants toxic to plants. This study examines the proteome changes in roots of actively growing Kandelia candel (L.) Druce when challenged with Cd. This mangrove-like species proliferates in estuaries and bays and is a potential choice for phytoremediation of Cd. A total of 53 proteins were up- or down-regulated following a short-term Cd treatment. The identities of the differentially expressed proteins were determined by MALDI-TOF/TOF. Approximately half of the up-regulated proteins are involved in oxidative response, including antioxidant enzymes, enzymes required for glutathione biosynthesis, enzymes in TCA and PPP cycles for generating ATP, NADH and NADPH. These results support the prediction that a prompt antioxidative response is necessary for the reduction of the oxidative stress caused by Cd and set the stage for further investigating of Cd up-regulated proteins in Kandelia candel.