Continuous Detection of pH-responsive Drug Delivery System in Cells in situ by Confocal Laser Scanning Microscopy

Mesoporous silica nanoparticles （MSN） were coated by pH-responsive polymer chitosan-poly （methacrylic acid） （CS-PMAA）. This nano drug delivery system showed good application prospects and the polymer-coated microspheres were promising site-specific anticancer drug delivery carriers in biomedical fie...

Full description

Saved in:
Bibliographic Details
Published in:Chinese journal of chemistry 2013-06, Vol.31 (6), p.787-793
Main Authors: Sun, Yang, Ran, Zhipeng, Tang, Hongyan, Li, Yong, Song, Wenshuang, Ren, Qingguang, Yang, Wuli, Kong, Jilie
Format: Article
Language:English
Subjects:
Cells
CLSM
continuous detection
drug delivery
Drug delivery systems
in situ
Nanoparticles
pH responsive nanoparticles
pH响应性
传递系统
原位
激光共聚焦显微镜
激光扫描共聚焦显微镜
细胞系
药物输送
连续检测
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mesoporous silica nanoparticles （MSN） were coated by pH-responsive polymer chitosan-poly （methacrylic acid） （CS-PMAA）. This nano drug delivery system showed good application prospects and the polymer-coated microspheres were promising site-specific anticancer drug delivery carriers in biomedical field. A continuous detection of pH-responsive drug delivery system in cells in situ, utilizing MSN/CS-PMAA composite microspheres, was pro- posed. Two kinds of different cell lines, tumor cell line （Hela） and normal somatic cells （293T）, were used to inves- tigate the behaviours of the drug loaded system in the cells. Conclusions could be drawn from the fluorescent im- ages obtained by confocal laser scanning microscopy （CLSM）, modified drug-loaded microspheres （MSN/CS-PMAA） were ingested into cells more easily, the uptake of DOX@FITC-MSN/CS-PMAA by HeLa/293T cells were performed at pH 7.4/pH 6.8, DOX was released during the ingestion process, fluorescence intensity de- creased with time because of efflux transport and photo-bleaching. Fluoresence detection by flow cytometry was performed as comparison. The continuous fluorescent observation in situ could be widely used in the pH-responsive releasing process of drug delivery system in the cells.
ISSN:1001-604X
1614-7065
DOI:10.1002/cjoc.201300113