EPR study of nitric oxide production in rat tissues under hypokinesia

EPR spectroscopy was used to study the intensity of nitric oxide (NO) production upon modeling 60-day progressive hypokinesia (restriction of motor activity) in rats and estimating the content of (DETC) 2 -Fe 2+ -NO complexes in heart and liver tissues. In 30 days of hypokinesia, there was a 2–3-fol...

Full description

Saved in:
Bibliographic Details
Published in:Biophysics (Oxford) 2013-03, Vol.58 (2), p.203-205
Main Authors: Gainutdinov, Kh. L., Andrianov, V. V., Iyudin, V. S., Yurtaeva, S. V., Jafarova, G. G., Faisullina, R. I., Sitdikov, F. G.
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biophysics
Complex Systems Biophysics
Kinetics
Nitric oxide
Physics
Physics and Astronomy
Rodents
Spectrum analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:EPR spectroscopy was used to study the intensity of nitric oxide (NO) production upon modeling 60-day progressive hypokinesia (restriction of motor activity) in rats and estimating the content of (DETC) 2 -Fe 2+ -NO complexes in heart and liver tissues. In 30 days of hypokinesia, there was a 2–3-fold increase in tissue NO. Administration of a nonspecific inhibitor of NO synthases, L-NAME, to hypokinetic rats prior to measurement decreased their NO level even below the untreated control. Our results show that the intensified NO production in hypokinesia is mainly due to NO synthases, rather than to the nitrite reductase pathway.
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350913020073